Kemeny D M, Lessof M H
Int Arch Allergy Appl Immunol. 1987;83(2):113-20.
We have measured in allergic and non-allergic beekeepers IgE and IgG antibodies to the bee venom allergens, phospholipase A2 (PLA2) and hyaluronidase (HYAL), by the radioallergosorbent test (RAST) and a 125I-radiolabelled antigen-binding assay. The absolute amount of IgG antibody in a reference serum was determined by saturation analysis using 125I-radiolabelled PLA2 and HYAL. Using monoclonal anti-IgE coated microtitre plates, the absolute amount of IgE antibody to the same antigen was also determined by saturation analysis. Regardless of the IgE response to the different allergens, IgG antibody concentrations to PLA2 were invariably higher than those to HYAL. In addition, the ratio of IgG to IgE antibody was higher for PLA2 (220:1) than for HYAL (10:1). Higher levels of IgG antibody to both allergens (especially HYAL) were found in those who had had prolonged exposure to bee stings. These data suggest that the level of IgG antibody produced is related to the dose administered, while the amount of IgE antibody may be regulated by other factors.
我们通过放射变应原吸附试验（RAST）和125I放射性标记抗原结合试验，检测了过敏和非过敏养蜂人针对蜂毒过敏原磷脂酶A2（PLA2）和透明质酸酶（HYAL）的IgE和IgG抗体。使用125I放射性标记的PLA2和HYAL，通过饱和分析确定参考血清中IgG抗体的绝对量。使用单克隆抗IgE包被的微量滴定板，同样通过饱和分析确定针对相同抗原的IgE抗体的绝对量。无论对不同过敏原的IgE反应如何，针对PLA2的IgG抗体浓度始终高于针对HYAL的浓度。此外，PLA2的IgG与IgE抗体的比值（220:1）高于HYAL的比值（10:1）。在长期接触蜂蜇的人群中，发现针对两种过敏原（尤其是HYAL）的IgG抗体水平更高。这些数据表明，产生的IgG抗体水平与给予的剂量有关，而IgE抗体的量可能受其他因素调节。
