Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
Institut für Ernährungs- und Lebensmittelwissenschaften, Lebensmittelmikrobiologie und -hygiene, Rheinische Friedrich-Wilhelms-Universität Bonn, Bonn, Germany.
Int J Syst Evol Microbiol. 2022 Jul;72(7). doi: 10.1099/ijsem.0.005450.
A Gram-stain-positive, aerobic and endospore-forming bacterial strain, isolated from the root surface of maize () was taxonomically studied. It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63 is a member of the genus , most closely related to the type strain of (98.61%), followed by (98.47 %). Detailed phylogenetic analysis based on the 16S rRNA gene and the 87 proteins conserved within the phylum placed the strain into the Cereus clade. The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of were 80.97, 81.45 and 26.30 %, respectively. The quinone system of strain JJ-63 consisted exclusively of menaquinone MK-7. The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid. Major fatty acids were iso- and anteiso-branched with the major compounds iso-C and iso-C. Also, the characteristic compounds C iso and C 10 were found. Physiological and biochemical characteristics allowed a further phenotypic differentiation of strain JJ-63 from the most closely related species. For this reason, JJ-63 represents a novel species of the genus , for which the name sp. nov. is proposed, with JJ-63 (=LMG 32091=CCM 9090=DSM 111827= CIP 111899) as the type strain.
一株革兰氏阳性、需氧、产芽孢的细菌菌株，从玉米的根表面分离出来，对其进行了分类学研究。基于 16S rRNA 基因序列相似性比较，可以清楚地表明，菌株 JJ-63 是芽孢杆菌属的一个成员，与 （98.61%）的模式株最为密切相关，其次是 （98.47%）。基于 16S rRNA 基因和该门内 87 个保守蛋白的详细系统发育分析，将该菌株归入蜡样芽孢杆菌群。与 （80.97%）、平均氨基酸同一性（81.45%）和数字 DNA-DNA 杂交值（26.30%）相比，平均核苷酸同一性对模式株的 。菌株 JJ-63 的醌类系统仅由menaquinone MK-7 组成。极性脂谱由主要成分双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油和一种未鉴定的糖脂组成。主要脂肪酸为异和 anteiso-支链，主要化合物为 iso-C 和 iso-C。此外，还发现了特征化合物 C iso 和 C 10。生理生化特性使 JJ-63 菌株与最密切相关的种进一步表型分化。因此，JJ-63 代表芽孢杆菌属的一个新种，提议命名为 sp. nov.，其模式株为 JJ-63（=LMG 32091=CCM 9090=DSM 111827=CIP 111899）。
