Kämpfer Peter, Lipski André, McInroy John A, Clermont Dominique, Lamothe Lucie, Glaeser Stefanie P, Criscuolo Alexis
Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
Institut für Ernährungs- und Lebensmittelwissenschaften, Lebensmittelmikrobiologie und -hygiene, Rheinische Friedrich-Wilhelms-Universität Bonn, Bonn, Germany.
Int J Syst Evol Microbiol. 2023 Apr;73(4). doi: 10.1099/ijsem.0.005808.
Two Gram-stain-positive, aerobic, endospore-forming bacterial strains, isolated from the rhizosphere of were studied for their detailed taxonomic allocation. Based on 16S rRNA gene sequence similarity comparisons, both strains JJ-7 and JJ-60 were shown to be members of the genus . Strain JJ-7 was most closely related to the type strains of (99.6 %) and (98.7 %), and strain JJ-60 to (99.5 %). The 16S rRNA gene sequence similarities to all other species were ≤98.4 %. Both strains JJ-7 and JJ-60 showed 97.6 % 16S rRNA gene sequence similarity between each other. Genomic comparisons showed that the average nucleotide identity and digital DNA-DNA hybridization values to next related type strain genomes were always <94 and <56 %, respectively. The polar lipid profiles of both strains contain a number of phospholipids including diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, which is in accord with the genus . The major quinone was MK-7 in both strains. Major fatty acids were iso- and anteiso-branched. Physiological and biochemical characteristics allowed a further phenotypic differentiation of strains JJ-7 and JJ-60 from the most closely related species. Thus, each strain represents a novel species of the genus , for which the names sp. nov. and sp. nov. are proposed, with JJ-7 (=CIP 111892=DSM 111785=LMG 32088=CCM 9087) and JJ-60 (=CIP 111894=DSM 111787=LMG 32090=CCM 9086) as the type strains, respectively.
对从[具体植物名称]根际分离得到的两株革兰氏阳性、需氧、产芽孢细菌菌株进行了详细的分类学定位研究。基于16S rRNA基因序列相似性比较，菌株JJ - 7和JJ - 60均被证明是[具体属名]属的成员。菌株JJ - 7与[具体种名1]（99.6%）和[具体种名2]（98.7%）的模式菌株关系最为密切，菌株JJ - 60与[具体种名3]（99.5%）关系最为密切。与所有其他[具体属名]物种的16S rRNA基因序列相似性均≤98.4%。菌株JJ - 7和JJ - 60彼此之间的16S rRNA基因序列相似性为97.6%。基因组比较表明，与最相近的模式菌株基因组的平均核苷酸同一性和数字DNA - DNA杂交值分别始终<94%和<56%。两株菌株的极性脂质谱均包含多种磷脂，包括双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺，这与[具体属名]属一致。两株菌株的主要醌均为MK - 7。主要脂肪酸为异支链和anteiso - 支链。生理生化特征使菌株JJ - 7和JJ - 60能够与最相近的物种进行进一步的表型区分。因此，每株菌株均代表[具体属名]属的一个新物种，分别提出名称[新物种名1] sp. nov.和[新物种名2] sp. nov.，模式菌株分别为JJ - 7（=CIP 111892 = DSM 111785 = LMG 32088 = CCM 9087）和JJ - 60（=CIP 111894 = DSM 111787 = LMG 32090 = CCM 9086）。
