Ururahy Mirian Saavedra, Curylofo-Zotti Fabiana Almeida, Galo Rodrigo, Nogueira Lucas Fabricio Bahia, Ramos Ana Paula, Corona Silmara Aparecida Milori
Department of Restorative Dentistry, Sao Paulo University, Café Avenue, Monte Alegre, Ribeirão Preto, SP, Brazil.
Department of Dentistry, Federal University of Jequitinhonha and Mucuri Valleys, Diamantina, MG, Brazil.
Arch Oral Biol. 2017 Mar;75:68-73. doi: 10.1016/j.archoralbio.2016.11.017. Epub 2016 Dec 2.
To assess the effect of chitosan, at concentrations of 2.5% and 5.0%, on the wettability of the eroded dentin, followed by analysis of surface morphology by SEM.
104 bovine dentin slabs were ground, polished and then immersed in 20mL of citric acid (pH=3.2) under continuous stirring for 2h. Specimens were randomly divided according to the dentin substrate: sound and eroded, and then, subdivided into 4 groups (n=10): without rewetting (control), 1% acetic acid, 2.5% chitosan and 5.0% chitosan. Then, a drop of the adhesive system Single Bond 2 (3M) was deposited onto surface of each specimen. The contact angle between dentin surface and the adhesive system was measured by using a goniometer. The other 24 specimens were subjected to analysis under SEM. Statistical analysis was performed using the normality test (Kolmogorov-Smirnov) and Analysis of Variance (ANOVA) (p>0.05).
No differences were found between the angles produced on the eroded dentin rewetting with chitosan at the concentrations of 2.5% and 5%.
The chitosan, regardless of the concentration used, did not influence the eroded dentin wettability. Through SEM analysis, it was found particles of chitosan deposited on the surface and within the dentinal tubules.
评估浓度为2.5%和5.0%的壳聚糖对侵蚀性牙本质润湿性的影响，随后通过扫描电子显微镜（SEM）分析表面形态。
将104块牛牙本质片研磨、抛光，然后在持续搅拌下浸入20mL柠檬酸（pH = 3.2）中2小时。根据牙本质基质将标本随机分组：完好的和侵蚀性的，然后再细分为4组（n = 10）：不进行再湿润（对照组）、1%乙酸、2.5%壳聚糖和5.0%壳聚糖。然后，在每个标本表面滴一滴粘结系统Single Bond 2（3M）。使用测角仪测量牙本质表面与粘结系统之间的接触角。另外24个标本进行扫描电子显微镜分析。使用正态性检验（柯尔莫哥洛夫-斯米尔诺夫检验）和方差分析（ANOVA）（p>0.05）进行统计分析。
在侵蚀性牙本质上用2.5%和5%浓度的壳聚糖进行再湿润所产生的角度之间未发现差异。
壳聚糖，无论使用何种浓度，均不影响侵蚀性牙本质的润湿性。通过扫描电子显微镜分析，发现壳聚糖颗粒沉积在表面和牙本质小管内。
