长链非编码RNA在结肠腺癌中的表达特征及其对患者生存和预后判断的潜在价值：基于癌症基因组图谱数据库的生物信息学分析
Expression characteristics of long non-coding RNA in colon adenocarcinoma and its potential value for judging the survival and prognosis of patients: bioinformatics analysis based on The Cancer Genome Atlas database.
作者信息
Li Ruofan, Gao Xu, Sun Haitao, Sun Lixin, Hu Xiaojian
机构信息
Department of General Surgery, Beijing Luhe Hospital Capital Medical University, Beijing, China.
Department of Urology, Second Affiliated Hospital of Xi'an Medical College, Xi'an, China.
出版信息
J Gastrointest Oncol. 2022 Jun;13(3):1178-1187. doi: 10.21037/jgo-22-384.
BACKGROUND
To investigate the expression characteristics of long non-coding RNA (lncRNA) in colon adenocarcinoma (COAD) and its potential value in predicting the prognosis of patient survival.
METHODS
We downloaded COAD-related RNA sequencing (RNA-seq) data and patient survival data from The Cancer Genome Atlas (TCGA). The data were analyzed for lncRNA expression differences, subjected to Cox regression analysis for survival rate, and Kaplan-Meier (KM) survival curves were plotted to analyze the role of the key genes related to prognostic survival by pathway enrichment analysis.
RESULTS
The data of 494 COAD clinical samples from TCGA were analyzed; 204 lncRNAs were differentially expressed, 156 were up-regulated, and 48 were down-regulated. The 10 genes with the most significant expression differences were , , , , , , , , , and . Cox regression analysis of 204 lncRNA genes showed that 23 lncRNA genes with significant effects on the prognosis and survival rate of COAD patients were obtained when P<0.05 was used as the threshold. With P≤0.001 as the threshold, the KM curves of 4 genes (, , , ) were statistically significant (P<0.05). The gene was selected for Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and it was revealed that the inheritance of -regulated gene expression was closely related to tumor development, such as collagen-containing extracellular matrix, organogenesis, activity of membrane protein receptors, and ion channel activity. The signaling pathways regulated by were also closely related to tumors, such as neuroactive ligand-receptor interaction, the PI3K-AKT signaling pathway, calcium signaling pathway, and protein digestion and absorption.
CONCLUSIONS
In COAD, lncRNA is differentially expressed and plays an important role in the disease regulation. It has potential application value in the diagnosis, targeted therapy, and prognosis of COAD patients.
背景
探讨长链非编码RNA（lncRNA）在结肠腺癌（COAD）中的表达特征及其预测患者生存预后的潜在价值。
方法
从癌症基因组图谱（TCGA）下载COAD相关的RNA测序（RNA-seq）数据和患者生存数据。对数据进行lncRNA表达差异分析，进行生存率的Cox回归分析，并绘制Kaplan-Meier（KM）生存曲线，通过通路富集分析来分析与预后生存相关的关键基因的作用。
结果
分析了来自TCGA的494例COAD临床样本数据；204个lncRNAs差异表达，其中156个上调，48个下调。表达差异最显著的10个基因分别是 ， ， ， ， ， ， ， ， ，和 。对204个lncRNA基因进行Cox回归分析，以P<0.05为阈值时，得到23个对COAD患者预后和生存率有显著影响的lncRNA基因。以P≤0.001为阈值时，4个基因（ ， ， ， ）的KM曲线具有统计学意义（P<0.05）。选择基因 进行基因本体论（GO）功能和京都基因与基因组百科全书（KEGG）通路富集分析，结果显示 调控的基因表达遗传与肿瘤发生密切相关，如含胶原蛋白的细胞外基质、器官发生、膜蛋白受体活性和离子通道活性。 调控的信号通路也与肿瘤密切相关，如神经活性配体-受体相互作用、PI3K-AKT信号通路、钙信号通路以及蛋白质消化和吸收。
结论
在COAD中，lncRNA差异表达并在疾病调控中发挥重要作用。它在COAD患者的诊断、靶向治疗和预后方面具有潜在的应用价值。
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