Liu Haoyu, Liu Dawei, Li Zhipeng
Queen Mary School, Medical College, Nanchang University, Nanchang, China.
Biological Science, School of Biological and Chemical Science, Queen Mary University of London, London, UK.
J Gastrointest Oncol. 2023 Apr 29;14(2):824-832. doi: 10.21037/jgo-23-217.
To investigate the expression characteristics of ectodermal-neural cortex 1 () in gastrointestinal tumors and its potential value in judging the prognosis of patient survival.
RNA sequence (RNA-seq) data and patient survival data related to stomach adenocarcinoma (STAD) and colon adenocarcinoma (COAD) in gastric cancer and colon cancer from The Cancer Genome Atlas (TCGA) were downloaded for expression difference analysis and Cox survival regression analysis. A Kaplan-Meier (KM) survival curve was plotted to analyze the tumor invasion level of patients with different expression levels, and the main influencing pathways of were analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and protein network analysis.
The data of 405 STAD samples and 494 COAD clinical samples of TCGA were analyzed, and it was found that the expression of in tumor tissues of patients with both types of cancer was significantly higher than that in normal tissues, with Log fold change values of 1.97 and 2.06, respectively, P<0.001. Cox analysis revealed that the high expression of was not significantly correlated with the prognosis and survival time of patients with gastric cancer and patients with colon cancer: overall survival (OS) hazard ratio (HR): 1.039, 95% confidence interval (CI): 0.890-1.213, P=0.627 for gastric cancer, OS HR: 0.886, 95% CI: 0.702-1.111, P=0.306 for colon cancer. KEGG pathway enrichment analysis of gene revealed that was mainly involved in neuroactive ligand-receptor interaction. The high expression of was associated with various immune cells and different cells such as basophils, CD4 memory T cells, CD4 TEM, and MV endothelial cells in gastric and colon cancers. The results of protein interaction network analysis suggested that may be involved in regulating neurite formation and neural crest cell differentiation.
expression is elevated in both gastric and colon cancers, and ENC1 is associated with various immune cells and different cells such as basophils, CD4 memory T cells, CD4 TEM, and MV endothelial cells in both gastric and colon cancers, but does not affect the survival and prognosis of patients.
探讨外胚层神经皮质1（ENC1）在胃肠道肿瘤中的表达特征及其在判断患者生存预后中的潜在价值。
下载来自癌症基因组图谱（TCGA）的胃癌和结肠癌中与胃腺癌（STAD）和结肠腺癌（COAD）相关的RNA序列（RNA-seq）数据和患者生存数据，进行表达差异分析和Cox生存回归分析。绘制Kaplan-Meier（KM）生存曲线，分析不同ENC1表达水平患者的肿瘤侵袭程度，并通过京都基因与基因组百科全书（KEGG）富集分析和蛋白质网络分析，分析ENC1的主要影响途径。
分析了TCGA的405例STAD样本和494例COAD临床样本的数据，发现两种癌症患者肿瘤组织中ENC1的表达均显著高于正常组织，Log倍变化值分别为1.97和2.06，P<0.001。Cox分析显示，ENC1的高表达与胃癌患者和结肠癌患者的预后及生存时间无显著相关性：胃癌总生存（OS）风险比（HR）：1.039，95%置信区间（CI）：0.890-1.213，P=0.627；结肠癌OS HR：0.886，95%CI：0.702-1.111，P=0.306。基因ENC1的KEGG通路富集分析显示，ENC1主要参与神经活性配体-受体相互作用。ENC1的高表达与胃癌和结肠癌中的各种免疫细胞以及不同的基质细胞如嗜碱性粒细胞、CD4记忆T细胞、CD4 TEM和MV内皮细胞相关。ENC1蛋白质相互作用网络分析结果表明，ENC1可能参与调节神经突形成和神经嵴细胞分化。
ENC1在胃癌和结肠癌中表达均升高，且在胃癌和结肠癌中，ENC1均与各种免疫细胞以及不同的基质细胞如嗜碱性粒细胞、CD4记忆T细胞、CD4 TEM和MV内皮细胞相关，但不影响患者的生存和预后。
