Department of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul, Republic of Korea.
Transdisciplinary Major in Learning Health Systems, Department of Healthcare Sciences, Graduate School, Korea University, Seoul, Republic of Korea.
Pharm Biol. 2022 Dec;60(1):1341-1348. doi: 10.1080/13880209.2022.2096076.
The sleep-promoting activity of Gaertn. (Nymphaeaceae) alkaloids in leaves or seeds are well known. However, the sleep-promoting activity of the lotus rhizome (LE), which is used mainly as food, has not yet been evaluated.
We investigated the sleep-promoting activity of LE water extract.
Institute of Cancer Research (ICR) mice ( = 8) were subject to a pentobarbital-induced sleep test to assess changes in sleep latency and duration following the administration of LE (80-150 mg/kg). In addition, electroencephalography analysis was performed to determine the sleep quality after LE treatment as well as the sleep recovery effect of LE using a caffeine-induced insomnia SD rat model. Real-time PCR and western blot analysis were performed to investigate the expression of neurotransmitter receptors, and the GABA receptor antagonists were used for receptor binding analysis.
An oral administration of 150 mg/kg LE significantly increased sleep duration by 24% compared to the control. Furthermore, LE increased nonrapid eye movement (NREM) sleep by increasing theta and delta powers. In the insomnia model, LE increased sleep time by increasing NREM sleep. Moreover, treatment with picrotoxin and flumazenil decreased the sleep time by 33% and 23%, respectively, indicating an involvement of the GABA receptor in the sleep-enhancing activity of LE. The expression of GABA receptors and the concentration of GABA in the brain were increased by LE.
The results suggest that the sleep-promoting activity of LE was via the GABA receptor. Collectively, these data show that LE may promote sleep.
睡莲科（Nymphaeaceae）白果的叶子或种子具有促进睡眠的活性。然而，作为主要食物的莲藕根茎（LE）的促眠活性尚未得到评估。
研究 LE 水提取物的促眠活性。
用戊巴比妥钠诱导睡眠试验检测 LE（80-150mg/kg）给药后对睡眠潜伏期和持续时间的影响，评估 ICR 小鼠的睡眠促进活性。此外，进行脑电图分析以确定 LE 治疗后的睡眠质量，并使用咖啡因诱导的失眠 SD 大鼠模型评估 LE 的睡眠恢复作用。采用实时 PCR 和 Western blot 分析研究神经递质受体的表达，并使用 GABA 受体拮抗剂进行受体结合分析。
与对照组相比，口服 150mg/kg LE 可使睡眠持续时间显著增加 24%。此外，LE 通过增加θ波和δ波功率增加非快速眼动（NREM）睡眠。在失眠模型中，LE 通过增加 NREM 睡眠来增加睡眠时间。此外，用印防己毒素和氟马西尼处理可分别使睡眠时间减少 33%和 23%，表明 GABA 受体参与了 LE 的促眠活性。LE 增加了大脑中 GABA 受体的表达和 GABA 的浓度。
这些结果表明，LE 的促眠活性是通过 GABA 受体介导的。综上所述，这些数据表明 LE 可能具有促进睡眠的作用。
