Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, PR China; Key Laboratory of Environment Protection, Soil ecological protection and pollution control, Sichuan University & Department of Ecology and Environment of Sichuan, Chengdu 610065, Sichuan, PR China.
Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, PR China.
J Hazard Mater. 2021 Aug 15;416:125814. doi: 10.1016/j.jhazmat.2021.125814. Epub 2021 Apr 6.
Pleurotus has great potential for heavy metal mycoremediation. Using comparative transcriptome analysis, the response of Pleurotus ostreatus and Pleurotus cornucopiae under Cd contamination was evaluated. P. ostreatus and P. cornucopia accumulated 0.34 and 0.46 mg/g Cd in mycelium, respectively. Cd removal elevated with its concentration elevation, which reached 56.47% and 54.60% for P. ostreatus and P. cornucopia with Cd at 20 mg/L. Low-level Cd (≤ 1 mg/L) had no significant influence on either fungus, while varied response was observed under high-level Cd. 705 differentially expressed genes (DEGs) were identified in P. cornucopia at Cd1 and Cd20, whereas 12,551 DEGs in P. ostreatus. Differentially regulated functional categories and pathways were also identified. ATP-binding cassette transporters were involved in Cd transport in P. cornucopia, whereas the endocytosis and phagosome pathways were more enhanced in P. ostreatus. 26 enzymes including peroxisomal enzymes catalase and superoxide dismutase were upregulated in P. ostreatus, whereas only cytosolic catalase was overexpressed in P. cornucopia, suggesting their different Cd detoxification pathways. Also, the mitogen-activated protein kinase signaling pathway involved in Cd resistance in both species instead of glutathione metabolism, although more active in P. ostreatus. These findings provided new insight into the molecular mechanism of mycoremediation and accumulator screening.
平菇具有很强的重金属生物修复潜力。通过比较转录组分析，评估了糙皮侧耳和美味侧耳在镉污染下的反应。糙皮侧耳和美味侧耳的菌丝体分别积累了 0.34 和 0.46mg/g 的 Cd。随着 Cd 浓度的升高，其去除率也随之升高，当 Cd 浓度为 20mg/L 时，糙皮侧耳和美味侧耳的去除率分别达到 56.47%和 54.60%。低浓度 Cd（≤1mg/L）对两种真菌均无显著影响，而高浓度 Cd 下则表现出不同的反应。在 Cd1 和 Cd20 下，鉴定出糙皮侧耳中有 705 个差异表达基因（DEGs），而在糙皮侧耳中有 12551 个 DEGs。还鉴定出了差异调节的功能类别和途径。在糙皮侧耳中，ATP 结合盒转运蛋白参与了 Cd 的转运，而内吞作用和吞噬体途径在糙皮侧耳中得到了更加强化。26 种酶，包括过氧化物酶体酶过氧化氢酶和超氧化物歧化酶，在糙皮侧耳中上调，而只有细胞质过氧化氢酶在美味侧耳中过表达，表明它们有不同的 Cd 解毒途径。此外，参与两种物种 Cd 抗性的丝裂原激活蛋白激酶信号通路而不是谷胱甘肽代谢，尽管在糙皮侧耳中更为活跃。这些发现为生物修复和积累筛选的分子机制提供了新的见解。
