Sarp Gokhan, Yilmaz Erkan
Department of Analytical Chemistry, Faculty of Pharmacy, Erciyes University, 38050 Kayseri, Turkey.
ERNAM-Nanotechnology Research and Application Center, Erciyes University, 38039 Kayseri, Turkey.
ACS Omega. 2022 Jun 27;7(27):23223-23233. doi: 10.1021/acsomega.2c01311. eCollection 2022 Jul 12.
In this period when environmental pollution has become uncontrollable, the removal of drug active substances reaching the environment and the analysis of drug active substances in different matrix environments are important for both living life and a sustainable environment. Therefore, the production of multifunctional materials that can be used in these two different processes has gained importance in the literature. Based on this thought, in this study, a g-CN@TiO@FeO multifunctional nanohybrid material was synthesized and used for magnetic solid-phase extraction (MSPE) and photocatalytic degradation of trimethoprim and isoniazid, used together in tuberculosis treatment. All analyses were performed by high-performance liquid chromatography using a diode-array detection (HPLC-DAD) system. The synthesized material was characterized by X-ray diffraction spectroscopy (XRD), Raman spectroscopy, Fourier transform infrared (FTIR) spectroscopy, Brunauer-Emmett-Teller (BET) method, ζ-potential analysis, field-emission scanning electron microscopy (FE-SEM), and energy-dispersive X-ray spectroscopy (EDX). Important analytical parameters for the MSPE method such as the pH value of the sample solution, the volume of the sample solution, the amount of the sorbent, the type and volume of the elution solvent, and extraction time were optimized. The optimized MSPE method was then applied to different environmental waters and pharmaceutical samples. The recovery percentages for these samples were found to be between 95 and 107%. For trimethoprim and isoniazid, the limit of detections (LODs) were 0.055 and 0.145 and the limit of quantifications (LOQs) were 0.167 and 0.439 ng·mL, respectively. It was observed that ∼100% of trimethoprim and isoniazid active components were photocatalytically removed from the g-CN@TiO@FeO nanohybrid material in ∼120 min under UV light.
在环境污染已变得无法控制的这一时期，去除进入环境中的药物活性物质以及分析不同基质环境中的药物活性物质，对于生物生存和可持续环境而言都至关重要。因此，能够用于这两个不同过程的多功能材料的生产在文献中已变得至关重要。基于这一想法，在本研究中，合成了一种g-CN@TiO@FeO多功能纳米杂化材料，并将其用于甲氧苄啶和异烟肼的磁性固相萃取（MSPE）及光催化降解，这两种药物共同用于结核病治疗。所有分析均通过使用二极管阵列检测（HPLC-DAD）系统的高效液相色谱法进行。通过X射线衍射光谱（XRD）、拉曼光谱、傅里叶变换红外（FTIR）光谱、布鲁诺尔-埃米特-泰勒（BET）法、ζ电位分析、场发射扫描电子显微镜（FE-SEM）和能量色散X射线光谱（EDX）对合成材料进行了表征。对MSPE方法的重要分析参数，如样品溶液的pH值、样品溶液的体积、吸附剂的量、洗脱溶剂的类型和体积以及萃取时间进行了优化。然后将优化后的MSPE方法应用于不同的环境水样和药物样品。这些样品的回收率在95%至107%之间。对于甲氧苄啶和异烟肼，检测限（LOD）分别为0.055和0.145，定量限（LOQ）分别为0.167和0.439 ng·mL。观察到在紫外光下，约120分钟内，约100%的甲氧苄啶和异烟肼活性成分从g-CN@TiO@FeO纳米杂化材料中被光催化去除。
