State University of Santa Cruz, Rodovia Jorge Amado, Km 16, Salobrinho, Ilhéus, Bahia, 45662-900, Brazil.
Pontifical Catholic University of Paraná, School of Life Sciences, Rua Imaculada Conceição, 1155, Prado Velho, Curitiba, Paraná, 80215-901, Brazil.
Microbiology (Reading). 2022 Jul;168(7). doi: 10.1099/mic.0.001216.
is one of the main pathogens that impacts swine production. Given the need for methods for its control, the effect of lactic acid bacteria (LAB) and their metabolites against F4 was evaluated through cell culture and microbiological analysis. The strains 5.2, 6.2, and 7.1 were selected. To evaluate the action of their metabolites, lyophilized cell-free supernatants (CFS) were used. The effect of CFS was evaluated in HT-29 intestinal lineage cells; in inhibiting the growth of the pathogen in agar; and in inhibiting the formation of biofilms. The bioprotective activity of LAB was evaluated via their potential for autoaggregation and coaggregation with . The CFS did not show cytotoxicity at lower concentrations, except for 5.2 CFS, which is responsible for cell proliferation at doses lower than 10 mg ml. The CFS were also not able to inhibit the growth of F4 in agar; however, the CFS of 7.1 resulted in a significant decrease in biofilm formation at a dose of 40 mg ml. Regarding LAB, their direct use showed great potential for autoaggregation and coaggregation , thus suggesting possible effectiveness in animal organisms, preventing fixation and proliferation. New tests are needed to evaluate lower doses of CFS to control biofilms and confirm the bioprotective potential of LAB, and tests to assess the effect of LAB and their metabolites interacting with animal physiology.
是影响养猪生产的主要病原体之一。鉴于需要控制该病原体的方法，通过细胞培养和微生物分析评估了乳酸菌 (LAB) 及其代谢物对 F4 的影响。选择了菌株 5.2、6.2 和 7.1。为了评估其代谢物的作用，使用了冻干无细胞上清液 (CFS)。在 HT-29 肠系细胞中评估了 CFS 的作用；在琼脂中抑制病原体生长；并抑制生物膜形成。通过其与 的自动聚集和共聚能力评估 LAB 的生物保护活性。较低浓度的 CFS 没有显示细胞毒性，除了 5.2 CFS，其在低于 10mg/ml 的剂量下负责细胞增殖。CFS 也不能抑制琼脂中 F4 的生长；然而，7.1 的 CFS 在 40mg/ml 的剂量下导致生物膜形成显著减少。关于 LAB，它们的直接使用显示出很强的自动聚集和共聚潜力，因此表明在动物体内可能有效，防止固定和增殖。需要进行新的测试来评估较低剂量的 CFS 以控制生物膜并确认 LAB 的生物保护潜力，以及测试评估 LAB 及其代谢物与动物生理学相互作用的效果。
