State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; College of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230026, China.
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
J Hazard Mater. 2022 Oct 5;439:129594. doi: 10.1016/j.jhazmat.2022.129594. Epub 2022 Jul 15.
Bacterial biofilm is notorious for causing chronic infections, whose antibiotic treatment is bringing about severe multidrug resistance and environmental contamination. Stimuli-responsive nanocarriers have become encouraging materials to combat biofilm infections with high efficiency and low side effect. Herein, a charge-switchable and pH-responsive nanocomplex is fabricated via a facile aqueous one-pot zeolitic imidazolate framework-8 (ZIF-8) encapsulation of proteinase K (PK) and photosensitizer Rose Bengal (RB), for enzymatic and photodynamic therapies (PDT) against biofilm infections. Once encountering in acidic microenvironment, the surface charge of nanocomplex can switch self-adaptively from negative to positive, hence remarkably facilitating the biofilm penetration of nanocomplex. After acid-induced decomposition of nanocomplex, the released PK degrades biofilm matrix and loosens its structure, promoting diffusion of RB inside the biofilm. Afterwards, upon visible light illumination, the RB generates highly reactive oxygen species (ROS), which can readily and efficiently kill the remained bacteria even in the biofilm core. The charge-assisted penetration makes PK and RB fully functional, resulting in a cooperative effect concerning high biofilm eradication capacity, as testified by biofilm models both in vitro and in vivo. The green synthesis and good therapeutic performance of the nanocomplex manifests its considerable potential as a nontoxic and effective platform for biofilm treatment.
细菌生物膜以引起慢性感染而臭名昭著，其抗生素治疗导致了严重的多药耐药性和环境污染。刺激响应型纳米载体已成为一种有前途的材料，可以高效低副作用地对抗生物膜感染。在此，通过简便的水相一锅法将蛋白酶 K (PK) 和光敏剂 Rose Bengal (RB) 包封到沸石咪唑酯骨架-8 (ZIF-8) 中，制备了一种可转换电荷和 pH 响应的纳米复合物，用于针对生物膜感染的酶和光动力疗法 (PDT)。一旦遇到酸性微环境，纳米复合物的表面电荷可以自适应地从负电荷转变为正电荷，从而显著促进纳米复合物对生物膜的穿透。在纳米复合物的酸诱导分解后，释放的 PK 降解生物膜基质并使其结构松散，促进 RB 在生物膜内部的扩散。之后，在可见光照射下，RB 会产生高活性氧 (ROS)，即使在生物膜核心，也能轻易且高效地杀死残留的细菌。电荷辅助穿透使 PK 和 RB 充分发挥功能，在体外和体内的生物膜模型中都证明了其具有很高的生物膜清除能力的协同作用。纳米复合物的绿色合成和良好的治疗性能表明其作为一种无毒且有效的生物膜处理平台具有很大的潜力。
