College of Chemistry, Liaoning University, No. 66 Chongshan Middle Road, Shenyang 110036, China.
Ecology and Environmental Monitoring Center of Jilin Province, Changchun 130011, China.
ACS Appl Mater Interfaces. 2024 Apr 3;16(13):15959-15969. doi: 10.1021/acsami.4c00844. Epub 2024 Mar 21.
Perfluorooctanesulfonate (PFOS), an emerging organic contaminant, necessitates robust on-site detection strategies to safeguard human health and ecological balance. This study introduces a novel point-of-care testing (POCT) platform, combining a hydrogel kit with nanozymes and smartphone technology, for the highly sensitive detection of PFOS. The strategy utilizes copper-substituted cobalt-based Prussian blue analogue nanoboxes (CuCo-PBA NBs), which exhibit intricate hollow structures and remarkable peroxidase-like catalytic activity, efficiently catalyzing the oxidation of chromogenic substrates with hydrogen peroxide (HO). Density functional theory calculations elucidate the adsorption dynamics of HO on CuCo-PBA NBs, identifying the factors that improve the catalytic efficiency. The colorimetric POCT platform, integrating the hydrogel kit with a smartphone interface, demonstrates practical utility and achieves a detection limit of 1.43 × 10 mol L for PFOS. This research not only presents a new nanozyme design for PFOS detection in diverse matrices, such as lake water, whole blood, urine, and milk, but also paves the way for developing a portable and efficient POCT platform for a variety of emerging contaminants.
全氟辛烷磺酸（PFOS）作为一种新兴的有机污染物，需要强大的现场检测策略来保护人类健康和生态平衡。本研究引入了一种新的即时检测（POCT）平台，该平台结合了水凝胶试剂盒、纳米酶和智能手机技术，用于高度敏感地检测 PFOS。该策略利用铜取代的钴基普鲁士蓝类似物纳米盒（CuCo-PBA NBs），其具有复杂的空心结构和显著的过氧化物酶样催化活性，可高效催化过氧化氢（HO）氧化显色底物。密度泛函理论计算阐明了 HO 在 CuCo-PBA NBs 上的吸附动力学，确定了提高催化效率的因素。将水凝胶试剂盒与智能手机接口集成的比色 POCT 平台具有实际应用价值，对 PFOS 的检测限达到 1.43×10-9 mol L-1。本研究不仅为不同基质（如湖水、全血、尿液和牛奶）中的 PFOS 检测提出了一种新的纳米酶设计，还为开发各种新兴污染物的便携式高效 POCT 平台铺平了道路。
