Wei Simin, Tian Qiming, Zhao Xin, Liu Xiaopan, Husien Hosameldeen Mohamed, Liu Mingjiang, Bo Ruonan, Li Jingui
School of Veterinary Medicine, Yangzhou University, Yangzhou 225009, P. R. China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, P. R. China.
ACS Infect Dis. 2022 Aug 12;8(8):1618-1626. doi: 10.1021/acsinfecdis.2c00223. Epub 2022 Jul 19.
Extensive efforts are underway to overcome the rising prevalence of antibiotic resistance. Combination therapy may be a potential method to treat multidrug-resistant (MDR) bacterial infections. In this study, tea tree essential oil (TTO) nanoemulsion (nanoTTO) was used in combination with antibiotics to kill microbes. Results showed that nanoTTO enhanced the activities of multiple antibiotics against MDR (), and its antimicrobial activity was not changed against bacteria that were cultured in the presence of nanoTTO for 30 passages. Further studies to visualize and quantify intracellular antibiotics concentrations identified that nanoTTO increased the drug accumulation in MDR by disrupting outer and inner membranes and inhibiting the AcrAB-TolC efflux pump involved in membrane permeability. In addition, nanoTTO was effective in enhancing antibiotic efficacy in the infection model and mouse peritonitis model, suggesting a potential strategy against MDR bacterial infections.
人们正在付出巨大努力来应对日益普遍的抗生素耐药性问题。联合治疗可能是治疗多重耐药（MDR）细菌感染的一种潜在方法。在本研究中，茶树精油（TTO）纳米乳剂（nanoTTO）与抗生素联合使用以杀灭微生物。结果表明，nanoTTO增强了多种抗生素对MDR（）的活性，并且在存在nanoTTO的情况下培养30代的细菌中，其抗菌活性没有改变。进一步的研究通过可视化和量化细胞内抗生素浓度发现，nanoTTO通过破坏外膜和内膜并抑制参与膜通透性的AcrAB - TolC外排泵，增加了MDR中的药物积累。此外，nanoTTO在感染模型和小鼠腹膜炎模型中有效增强了抗生素疗效，提示了一种对抗MDR细菌感染的潜在策略。
