Molla Yitayeh Mequanint, Monie Wassihun Amanu
Department of Organic Chemistry, College of Natural and Computational Science, Debre Tabor University, Debre Tabor, Ethiopia.
Department of Biochemistry, College of Health Science, Debre Tabor University, Debre Tabor, Ethiopia.
Biochem Res Int. 2022 Jul 9;2022:4900917. doi: 10.1155/2022/4900917. eCollection 2022.
Herbal medication developed from natural resources has to have antibacterial and antioxidant effects. The aim of this research is to look at the chemical makeup of (. ) stem bark essential oil (EO), as well as the effectiveness of EO and extracts (chloroform, ethyl acetate, and methanol) against human pathogenic bacteria and their antioxidant activity. The GC-MS analysis identified 23 components, accounting for 98.7% of the total oil containing Methylene chloride (49.2%), sabinene (10.5%), 1-nonene (11.3%), Terpinen-4-ol (6.9%), Camphene (4.3%), -terpinene (3.6%), -phellandrene (2.9%) -myrcene (2.6%), 1,2,5-Oxadiazol-3-carboxamide, 4,4'-azobis-2,2'-dioxide (2.4%), -terpinene (1.9%), 1-Octanamine, N-methyl- (1.9%), -cymene (1.6%) as major components. The antibacterial efficacy of the EO and extracts (25, 50, 100, and 200 mg/ml) was demonstrated by the inhibitory zones (8.5 ± 0.47-23.3 ± 0.36 and 7.2 ± 0.25-22.0 ± 0.45 mm), respectively. The MIC values of the extracts and the EO were 120-150 and 240 to <1100 g/ml, respectively. The EO also demonstrated a significant antibacterial impact. The EO and methanolic extract had free radical scavenging activities with IC value, 13.8 ± 0.48 and 4.2 ± 0.04 g/ml, respectively. In comparison to the other extracts, the methanolic extract had the greatest phenolics (100.2 ± 0.13 g GAE/mg of dry extract) and flavonoid contents (112.1 ± 0.18 g CE/mg of dry extract).
由自然资源开发的草药必须具有抗菌和抗氧化作用。本研究的目的是研究（.）茎皮精油（EO）的化学成分，以及EO和提取物（氯仿、乙酸乙酯和甲醇）对人类病原菌的有效性及其抗氧化活性。气相色谱-质谱分析鉴定出23种成分，占总油含量的98.7%，其中二氯甲烷（49.2%）、桧烯（10.5%）、1-壬烯（11.3%）、萜品-4-醇（6.9%）、莰烯（4.3%）、γ-萜品烯（3.6%）、α-水芹烯（2.9%）、β-月桂烯（2.6%）、1,2,5-恶二唑-3-甲酰胺、4,4'-偶氮双-2,2'-二氧化物（2.4%）、γ-萜品烯（1.9%）、1-辛胺、N-甲基-（1.9%）、对异丙基甲苯（1.6%）为主要成分。EO和提取物（25、50、100和200毫克/毫升）的抑菌圈分别为（8.5±0.47-23.3±0.36和7.2±0.25-22.0±0.45毫米），证明了其抗菌效果。提取物和EO的最低抑菌浓度值分别为120-150和240至<1100微克/毫升。EO也显示出显著的抗菌作用。EO和甲醇提取物具有自由基清除活性，IC值分别为13.8±0.48和4.2±0.04微克/毫升。与其他提取物相比，甲醇提取物的酚类物质含量最高（100.2±0.13微克没食子酸当量/毫克干提取物），黄酮类含量最高（112.1±0.18微克儿茶素当量/毫克干提取物）。
