Chemical Composition and Antibacterial and Antioxidant Activities of Stem Bark Essential Oil and Extracts of .

Herbal medication developed from natural resources has to have antibacterial and antioxidant effects. The aim of this research is to look at the chemical makeup of (. ) stem bark essential oil (EO), as well as the effectiveness of EO and extracts (chloroform, ethyl acetate, and methanol) against human pathogenic bacteria and their antioxidant activity. The GC-MS analysis identified 23 components, accounting for 98.7% of the total oil containing Methylene chloride (49.2%), sabinene (10.5%), 1-nonene (11.3%), Terpinen-4-ol (6.9%), Camphene (4.3%), -terpinene (3.6%), -phellandrene (2.9%) -myrcene (2.6%), 1,2,5-Oxadiazol-3-carboxamide, 4,4'-azobis-2,2'-dioxide (2.4%), -terpinene (1.9%), 1-Octanamine, N-methyl- (1.9%), -cymene (1.6%) as major components. The antibacterial efficacy of the EO and extracts (25, 50, 100, and 200 mg/ml) was demonstrated by the inhibitory zones (8.5 ± 0.47-23.3 ± 0.36 and 7.2 ± 0.25-22.0 ± 0.45 mm), respectively. The MIC values of the extracts and the EO were 120-150 and 240 to <1100 g/ml, respectively. The EO also demonstrated a significant antibacterial impact. The EO and methanolic extract had free radical scavenging activities with IC value, 13.8 ± 0.48 and 4.2 ± 0.04 g/ml, respectively. In comparison to the other extracts, the methanolic extract had the greatest phenolics (100.2 ± 0.13 g GAE/mg of dry extract) and flavonoid contents (112.1 ± 0.18 g CE/mg of dry extract).