Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia.
Molecules. 2022 Jan 27;27(3):838. doi: 10.3390/molecules27030838.
Some species of , such as , are well-known as traditional Chinese medicine (TCM), and their pharmacological value was scientifically proven in modern days. However, is recognized as an oil palm pathogen, and its biological activity is scarcely reported. Hence, this study aimed to investigate the antibacterial properties of fruiting bodies, which formed by condensed mycelial, produced numerous and complex profiles of natural compounds. Extract was cleaned up with normal-phase SPE and its metabolites were analyzed using liquid chromatography-mass spectrometry (LCMS). From the disc diffusion and broth microdilution assays, strong susceptibility was observed in methicillin-resistant (MRSA) in elute fraction with zone inhibition of 41.08 ± 0.04 mm and MIC value of 0.078 mg mL. A total of 23 peaks were detected using MS, which were putatively identified based on their mass-to-charge ratio (), and eight compounds, which include aristolochic acid, aminoimidazole ribotide, lysine sulfonamide 11v, carbocyclic puromycin, fenbendazole, acetylcaranine, tigecycline, and tamoxifen, were reported in earlier literature for their antimicrobial activity. Morphological observation via scanning electron microscope (SEM), cell membrane permeability, and integrity assessment suggest extract induces irreversible damage to the cell membrane of MRSA, thus causing cellular lysis and death.
一些物种，如，被公认为传统中药（TCM），其药理学价值在现代得到了科学证实。然而，被认为是油棕病原体，其生物活性鲜有报道。因此，本研究旨在研究由浓缩菌丝体形成的子实体的抗菌特性，这些子实体产生了许多复杂的天然化合物。提取物用正相 SPE 进行净化，并用液相色谱-质谱联用仪（LCMS）分析其代谢物。从纸片扩散和肉汤微量稀释试验来看，洗脱部分对耐甲氧西林金黄色葡萄球菌（MRSA）表现出很强的敏感性，抑菌环抑制率为 41.08±0.04mm，MIC 值为 0.078mg/mL。使用 MS 检测到 23 个峰，根据质荷比（）推测它们的身份，并在先前的文献中报道了八种化合物，包括马兜铃酸、氨基咪唑核糖核苷酸、赖氨酸磺酰胺 11v、碳环嘌呤霉素、芬苯达唑、乙酰卡啉、替加环素和他莫昔芬，它们具有抗菌活性。扫描电子显微镜（SEM）观察形态学、细胞膜通透性和完整性评估表明，提取物诱导 MRSA 细胞膜不可逆损伤，导致细胞裂解和死亡。
