Larrieu Antoine, Brunoud Géraldine, Guérault Aurore, Lainé Stéphanie, Hennet Lauriane, Stigliani Arnaud, Gildea Iris, Just Jeremy, Soubigou-Taconnat Ludivine, Balzergue Sandrine, Davies Brendan, Scarpella Enrico, Helariutta Ykä, Parcy François, Vernoux Teva
Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, CNRS, INRAE, 69342 Lyon, France.
Univ. Grenoble Alpes, CNRS, CEA, INRAE, BIG-LPCV, 38000 Grenoble, France.
iScience. 2022 Jun 27;25(7):104683. doi: 10.1016/j.isci.2022.104683. eCollection 2022 Jul 15.
Coordinating growth and patterning is essential for eukaryote morphogenesis. In plants, auxin is a key regulator of morphogenesis implicated throughout development. Despite this central role, our understanding of how auxin coordinates cell fate and growth changes is still limited. Here, we addressed this question using a combination of genomic screens to delve into the transcriptional network induced by auxin at the earliest stage of flower development, prior to morphological changes. We identify a shoot-specific network suggesting that auxin initiates growth through an antagonistic regulation of growth-promoting and growth-repressive hormones, quasi-synchronously to floral fate specification. We further identify two DNA-binding One Zinc Finger (DOF) transcription factors acting in an auxin-dependent network that could interface growth and cell fate from the early stages of flower development onward.
协调生长和模式形成对于真核生物形态发生至关重要。在植物中，生长素是整个发育过程中涉及形态发生的关键调节因子。尽管生长素具有这一核心作用，但我们对其如何协调细胞命运和生长变化的理解仍然有限。在这里，我们通过基因组筛选的组合来解决这个问题，以深入研究在花发育的最早阶段，即在形态变化之前，由生长素诱导的转录网络。我们鉴定出一个茎特异性网络，表明生长素通过对促进生长和抑制生长的激素进行拮抗调节来启动生长，这与花命运的指定几乎同步。我们进一步鉴定出两个在生长素依赖性网络中起作用的DNA结合单锌指（DOF）转录因子，它们可以从花发育的早期阶段起连接生长和细胞命运。
