Kitajima Hiroaki, Komatsu Keiji, Matsuura Takanori, Ozawa Ryotaro, Saruta Juri, Taleghani Samira Rahim, Cheng James, Ogawa Takahiro
Weintraub Center for Reconstructive Biotechnology and the Division of Regenerative and Reconstructive Sciences, UCLA School of Dentistry, Los Angeles, CA.
J Prosthodont Res. 2023 Apr 12;67(2):288-299. doi: 10.2186/jpr.JPR_D_22_00015. Epub 2022 Jul 21.
A novel implant model consisting of meso-scale cactus-inspired spikes and nano-scale bone-inspired trabeculae was recently developed to optimize meso-scale roughness on zirconia. In this model, the meso-spike dimension had a significant impact on osteoblast function. To explore how different nano-textures impact this model, here we examined the effect of different nano-trabecula sizes on osteoblast function while maintaining the same meso-spike conformation.
Zirconia disks with meso-nano hybrid surfaces were created by laser etching. The meso-spikes were fixed to 40 μm high, whereas the nano-texture was etched as large and small trabeculae of average Feret diameter 237.0 and 134.1 nm, respectively. A polished surface was also prepared. Rat bone marrow-derived and human mesenchymal stromal cell-induced osteoblasts were cultured on these disks.
Hybrid rough surfaces, regardless of nano-trabecula dimension, robustly promoted the osteoblastic differentiation of both rat and human osteoblasts compared to those on polished surfaces. Hybrid surfaces with small nano-trabeculae further enhanced osteoblastic differentiation compared with large nano-trabeculae. However, the difference in osteoblastic differentiation between small and large nano-trabeculae was much smaller than the difference between the polished and hybrid rough surfaces. The nano-trabecula size did not influence osteoblast attachment and proliferation, or protein adsorption. Both hybrid surfaces were hydro-repellent. The atomic percentage of surface carbon was lower on the hybrid surface with small nano-trabeculae.
Small nano-trabeculae promoted osteoblastic differentiation more than large nano-trabeculae when combined with meso-scale spikes. However, the biological impact of different nano-trabeculae was relatively small compared with that of different dimensions of meso-spikes.
最近开发了一种新型植入物模型，其由中尺度仙人掌启发的尖刺和纳米尺度骨启发的小梁组成，以优化氧化锆上的中尺度粗糙度。在该模型中，中尺度尖刺尺寸对成骨细胞功能有显著影响。为了探究不同的纳米纹理如何影响该模型，我们在此研究了在保持相同中尺度尖刺构象的情况下，不同纳米小梁尺寸对成骨细胞功能的影响。
通过激光蚀刻制备具有中纳米混合表面的氧化锆圆盘。中尺度尖刺固定为40μm高，而纳米纹理分别蚀刻为平均费雷特直径237.0和134.1nm的大小不同的小梁。还制备了一个抛光表面。将大鼠骨髓来源的和成人间充质基质细胞诱导的成骨细胞培养在这些圆盘上。
与抛光表面相比，无论纳米小梁尺寸如何，混合粗糙表面均有力地促进了大鼠和人类成骨细胞的成骨分化。与大纳米小梁相比，具有小纳米小梁的混合表面进一步增强了成骨分化。然而，小纳米小梁和大纳米小梁之间的成骨分化差异远小于抛光表面和混合粗糙表面之间的差异。纳米小梁尺寸不影响成骨细胞的附着、增殖或蛋白质吸附。两种混合表面均具有疏水性。具有小纳米小梁的混合表面上的表面碳原子百分比更低。
与中尺度尖刺结合时，小纳米小梁比大纳米小梁更能促进成骨分化。然而，与不同尺寸的中尺度尖刺相比，不同纳米小梁的生物学影响相对较小。
