Technology Research Center, Wuliangye Yibin Co., Ltd., 150# Minjiang West Road, Cuiping District, Yibin, Sichuan, 644007, PR China.
Int J Syst Evol Microbiol. 2022 Jul;72(7). doi: 10.1099/ijsem.0.005464.
During an investigation of the diversity of airborne yeasts in a famous Chinese baijiu fermentation workshop, two yeast strains were isolated from the air of the Wuliangye 501# baijiu-making workshop in Yibin, Sichuan Province, PR China and subjected to taxonomic analysis. The results of phylogenetic analysis of two regions of the rRNA gene cluster, the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, indicated that these strains represented a novel species of the genus distinct from closely related species. The similarity between the novel species and the most closely related known species, , was 94.1 % for the D1/D2 LSU rRNA gene (30 substitutions and 12 indels out of 556 bp) and 95.6 % for the ITS region (9 substitutions and 6 indels out of 475 bp). In contrast with , this novel species was able to assimilate d-ribose, l-arabinose, d-arabinose, cellobiose, d-glucono-1,5-lactone, dl-lactate, citrate, 1,2-propanediol, 2,3-butanediol and ethanol but was unable to ferment raffinose or assimilate inulin. On the basis of the results of phylogenetic analysis and the physiological characteristics, these investigated strains represent a novel species of the genus , for which the name sp. nov. is proposed. Its holotype is CGMCC 20235, and the MycoBank number is MB840188.
在对中国著名白酒发酵车间空气中的 airborne yeasts 多样性进行调查时，从四川省宜宾市的 501 号五粮液白酒酿造车间空气中分离到两株酵母菌株，并进行了分类分析。rRNA 基因簇的两个区域（大亚基（LSU）rRNA 基因的 D1/D2 结构域和内部转录间隔区（ITS）区）的系统发育分析结果表明，这些菌株代表了一个与亲缘关系密切的种不同的新型种。新型种与最密切相关的已知种的相似性为 94.1%，对于 LSU rRNA 基因的 D1/D2 结构域（556bp 中有 30 个取代和 12 个插入）和 ITS 区（475bp 中有 9 个取代和 6 个插入）。与 相比，该新型种能够同化 d-核糖、l-阿拉伯糖、d-阿拉伯糖、纤维二糖、d-葡萄糖-1,5-内脂、dl-乳酸、柠檬酸、1,2-丙二醇、2,3-丁二醇和乙醇，但不能发酵棉子糖或同化菊粉。基于系统发育分析和生理特性的结果，这些研究菌株代表了一个新型种的属，建议将其命名为 。其模式菌株为 CGMCC 20235，MycoBank 编号为 MB840188。
