Culture Collection of Yeasts, Institute of Chemistry, Slovak Academy of Sciences,, Dúbravská cesta 9, 845 38 Bratislava, Slovakia.
Int J Syst Evol Microbiol. 2023 Feb;73(2). doi: 10.1099/ijsem.0.005719.
Our previous studies focused on the diversity of yeasts related to the aboveground parts of fruit trees, as well as the soil adjacent to these trees, located in the south-west of Slovakia. During these studies, we isolated two strains: CCY 11-1-1 from the blossoms of a peach tree () and CCY 11-1-2 from the soil adjacent to a pear tree (), both found in the Malé Zálužie locality. The sequences of the D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region were identical in both strains. They differed only by two nucleotide substitutions in the segment of the gene of translation elongation factor one alpha (). Phylogenetic analysis demonstrated that strains CCY 11-1-1 and CCY 11-1-2 formed a separate species in the clade of insect-associated members of the genus . The strains differed from the closest species by 23 nucleotide substitutions and 12 indels in the D1/D2 domain, more than 6 % in the ITS region (31 nt and 25 indels) and by 44 nt in the segment of . Therefore, these two strains are recognized as belonging to a novel species, for which we have proposed the name sp. nov., derived from the locality of their origin, Malé Zálužie. The type strain of sp. nov. is CCY 11-1-1.
我们之前的研究集中于与果树地上部分以及这些树附近土壤相关的酵母菌多样性，研究地点位于斯洛伐克西南部。在这些研究中，我们从一棵桃树的花朵（）中分离到一株 CCY 11-1-1 菌株，从一棵梨树附近的土壤（）中分离到一株 CCY 11-1-2 菌株，这两株菌均来自 Malé Zálužie 地区。这两个菌株的大亚基（LSU）核糖体 RNA 基因 D1/D2 结构域和内部转录间隔区（ITS）序列完全相同。它们仅在翻译延伸因子 1α 基因的一段（）中存在两个核苷酸取代的差异。系统发育分析表明，菌株 CCY 11-1-1 和 CCY 11-1-2 形成了一个单独的种，属于昆虫相关属的一个分支。这两个菌株与最接近的种 相比，在 D1/D2 结构域中有 23 个核苷酸取代和 12 个插入/缺失，ITS 区有超过 6%的差异（31 个核苷酸和 25 个插入/缺失），在 结构域的一段有 44 个核苷酸差异。因此，这两个菌株被认为属于一个新种，我们提议将其命名为 sp. nov.，源自其起源地 Malé Zálužie。 sp. nov. 的模式菌株为 CCY 11-1-1。
