Department of Surgery, Iwate Medical University School of Medicine, Yahaba, Iwate, Japan.
Division of Hepatology, Department of Internal Medicine, Iwate Medical University School of Medicine, Yahaba, Iwate, Japan.
PLoS One. 2022 Jul 21;17(7):e0271698. doi: 10.1371/journal.pone.0271698. eCollection 2022.
This study investigated whether liver damage severity relates to the mobilization of multilineage-differentiating stress-enduring (Muse) cells, which are endogenous reparative pluripotent stem cells, into the peripheral blood (PB) and whether the degree of mobilization relates to the recovery of liver volume following human liver surgery.
Forty-seven patients who underwent liver surgery were included in the present study. PB-Muse cells were counted before surgery, on postoperative days (PODs) 3 and on POD 7. Liver volume was measured using computed tomography before and after surgery.
The PB-Muse cell count increased after surgery. The number of PB-Muse cells before surgery was higher, but without statistical significance in the group with neoplasms than in the healthy group that included liver donors (p = 0.065). Forty-seven patients who underwent liver surgery were divided into major hepatic resection (MHR; hepatectomy of three or more segments according to the Couinaud classification, n = 22) and minor hepatic resection (mhr; hepatectomy of two segments or less according to the Couinaud classification, n = 25) groups. PB-Muse cells increased at high rates among MHR patients (p = 0.033). Except for complication cases, PB-Muse cells increased at higher rates in the group with advanced liver volume recovery (p = 0.043). The predictive impact of the rate of increase in PB-Muse cells on the recovery of liver volume was demonstrated by multivariate analysis (OR 11.0, p = 0.014).
PB-Muse cell mobilization correlated with the volume of liver resection, suggesting that the PB-Muse cell number reflects the degree of liver injury. Given that the degree of PB-Muse cell mobilization was related to liver volume recovery, PB-Muse cells were suggested to contribute to liver regeneration, although this mechanism remains unclear.
本研究旨在探讨肝损伤严重程度与多谱系分化应激耐受（Muse）细胞动员进入外周血（PB）之间的关系，Muse 细胞是内源性修复多能干细胞，以及动员程度与肝切除术后肝体积恢复之间的关系。
本研究纳入 47 例行肝切除术的患者。在术前、术后第 3 天和第 7 天分别计数 PB-Muse 细胞。在术前和术后使用计算机断层扫描测量肝体积。
手术后 PB-Muse 细胞计数增加。手术组患者术前 PB-Muse 细胞数量较高，但与包括肝供体在内的健康组相比，无统计学意义（p = 0.065）。47 例行肝切除术的患者分为大肝切除术（MHR；根据 Couinaud 分类行三个或更多节段肝切除术，n = 22）和小肝切除术（mhr；根据 Couinaud 分类行两个节段或更少肝切除术，n = 25）组。MHR 患者的 PB-Muse 细胞以较高的速度增加（p = 0.033）。除并发症病例外，肝体积恢复较好的患者 PB-Muse 细胞增加率较高（p = 0.043）。多变量分析显示，PB-Muse 细胞增加率对肝体积恢复的预测作用（OR 11.0，p = 0.014）。
PB-Muse 细胞动员与肝切除量相关，提示 PB-Muse 细胞数量反映了肝损伤程度。鉴于 PB-Muse 细胞动员程度与肝体积恢复有关，尽管其机制尚不清楚，但提示 PB-Muse 细胞有助于肝再生。
