State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
Plant Signal Behav. 2022 Dec 31;17(1):2100685. doi: 10.1080/15592324.2022.2100685.
Flowering at an appropriate time is crucial for plant development and reproduction. In , the flowering process is managed by a regulatory network composed of at least 6 independent pathways. As a core protein in flowering regulation, FLOWERING LOCUS T (FT) participates in almost all these pathways. ANKYRIN REPEAT-CONTAINING PROTEIN 2A (AKR2A) was initially discovered as a 14-3-3-interacting protein. It was then found to be involved in the transportation of chloroplast outer membrane proteins and the resistance to low-temperature stress. Here, we identified an null mutant with a delayed flowering phenotype. Through the quantitative real-time PCR (qRT-PCR) and bimolecular fluorescence complementation (BiFC) assays, we demonstrated that AKR2A modulates the flowering process through its interaction with FT.
在适当的时间开花对于植物的发育和繁殖至关重要。在拟南芥中,开花过程由至少 6 个独立途径组成的调控网络来调控。作为开花调控的核心蛋白,FLOWERING LOCUS T (FT) 参与了几乎所有这些途径。ANKYRIN REPEAT-CONTAINING PROTEIN 2A (AKR2A) 最初被发现是一个与 14-3-3 相互作用的蛋白。后来发现它参与了质体外膜蛋白的运输和抗低温胁迫。在这里,我们鉴定了一个 缺失突变体,其具有开花延迟的表型。通过定量实时 PCR(qRT-PCR)和双分子荧光互补(BiFC)实验,我们证明 AKR2A 通过与 FT 的相互作用来调节开花过程。