Laboratório de Doenças Infectocontagiosas da Faculdade de Medicina Veterinária da, Universidade Federal de Uberlândia (UFU), Av. Ceará, s/n. 38.4000.902, Uberlândia, MG, Brasil.
Faculdade de Medicina Veterinária do Centro, Universitário de Patos de Minas (UNIPAM), Patos de Minas, MG, Brasil.
Trop Anim Health Prod. 2022 Jul 22;54(4):238. doi: 10.1007/s11250-022-03243-2.
Genotyping methods have led to a better understanding of the epidemiology of Mycobacterium bovis (M. bovis) infection, and its transmission dynamics, as well as the possible phylogenetic relationships between Mycobacterium strains, thus making bovine tuberculosis control programs more efficient. The goal of this study was to characterize the main spoligotypes of M. bovis isolated from cattle in the State of Minas Gerais, Brazil. It was carried out in 28 municipalities of "Triângulo Mineiro" and "Alto Paranaíba" regions of the state. Viscera samples were obtained from 58 bovines positive for tuberculosis according to comparative cervical tests, and from another 100 bovines with lesions suggestive of tuberculosis, which were donated by the National Agricultural Laboratory of Pedro Leopoldo, Minas Gerais. Microbiological isolation was performed in Stonebrink medium, and molecular identification of mycobacteria was performed by PCR. Genotyping was performed using the spoligotyping method at the Agrobiotechnology and Molecular Biology Institute of National Agricultural Technology Institute-National Scientific and Technical Research Council, Buenos Aires, Argentina. Among the 158 viscera samples, we obtained 40 (25%) isolates of M. bovis, and detected 11 spoligotype patterns, with a predominance of SB1142 (37.5%), SB0121 (25.0%), and SB1145 (10.0%). Other standards, SB0295, SB1050, SB0881, SB1144, SB1802, SB0140, SB0120, and SB0849, varied from 2.5 to 7.5%, heterogeneously distributed among the municipalities. The presence of spoligotypes shared with other Brazilian states and different countries indicates their possible exchange through epidemiological relationships, such as the transit of live animals and/or genetic similarity between strains that share a common ancestor.
基因分型方法使我们更好地了解牛分枝杆菌（M. bovis）感染的流行病学及其传播动态，以及分枝杆菌菌株之间可能的系统发育关系，从而使牛结核病控制计划更加有效。本研究的目的是对巴西米纳斯吉拉斯州牛分枝杆菌分离株的主要 spoligotype 进行特征描述。该研究在该州的“Triângulo Mineiro”和“Alto Paranaíba”地区的 28 个城市进行。根据比较颈试验，从 58 头经检测为结核病阳性的牛中获得内脏样本，从另外 100 头疑似结核病病变的牛中获得内脏样本，这些牛是由米纳斯吉拉斯州佩德罗·莱奥波尔多国家农业实验室捐赠的。在 Stonebrink 培养基中进行微生物分离，并用 PCR 进行分枝杆菌的分子鉴定。在阿根廷国家农业技术研究所-国家科学技术研究理事会的农业生物技术和分子生物学研究所，使用 spoligotyping 方法进行基因分型。在 158 个内脏样本中，我们获得了 40 株（25%）牛分枝杆菌分离株，并检测到 11 种 spoligotype 模式，其中 SB1142（37.5%）、SB0121（25.0%）和 SB1145（10.0%）占主导地位。其他标准，SB0295、SB1050、SB0881、SB1144、SB1802、SB0140、SB0120 和 SB0849，占 2.5%至 7.5%，在各市镇之间呈不均匀分布。与巴西其他州和不同国家共享 spoligotype 的存在表明它们可能通过流行病学关系（如活畜的过境和/或具有共同祖先的菌株之间的遗传相似性）进行交换。
