Key Laboratory of Public Health Safety of Hebei Province, College of Public Health, School of Life Sciences, Hebei University, Baoding 071002, China.
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China.
Food Chem. 2022 Dec 1;396:133690. doi: 10.1016/j.foodchem.2022.133690. Epub 2022 Jul 15.
Polycyclic aromatic hydrocarbons (PAHs) posed a serious threat to food safety and human health due to long-term emission. In this work, a new method was established using phloroglucinol-urotropine porous polymer (PU-PP) in a pipette tip for solid phase extraction (PT-SPE) for the first time and used prior to determination of four PAHs (phenanthrene, anthracene, fluoranthene, and pyrene) in lotus roots. Synthesis of the PU-PP adsorbent was green compared with alternatives; urotropine was used as a cross-linker and ethanol-water as the solvent. PU-PP-based PT-SPE had the advantages of low solvent consumption, good purification, practicability, stability, and low-cost. The proposed pre-purification method offered low limits of detection (0.09-0.28 ng/g) and good recoveries (84.6-114.3 %, RSDs ≤ 5.6 %) for determination of the four PAHs, which were detected at trace concentrations in samples. This new method provides an alternative for monitoring trace pollutants in aquatic plant ingredients.
多环芳烃（PAHs）由于长期排放，对食品安全和人体健康构成了严重威胁。本工作首次建立了一种使用间苯三酚-六亚甲基四胺多孔聚合物（PU-PP）在微量管内固相萃取（PT-SPE）的新方法，并用于测定莲藕中的四种 PAHs（菲、蒽、荧蒽和芘）。与替代方法相比，PU-PP 吸附剂的合成更为绿色环保；六亚甲基四胺用作交联剂，乙醇-水用作溶剂。基于 PU-PP 的 PT-SPE 具有溶剂消耗低、净化效果好、实用性强、稳定性好、成本低等优点。该预净化方法为四种 PAHs 的测定提供了较低的检测限（0.09-0.28ng/g）和良好的回收率（84.6-114.3%，RSDs≤5.6%），这些 PAHs 在样品中以痕量浓度存在。这种新方法为监测水生植物成分中的痕量污染物提供了一种替代方法。
