Department of Biology, Shahed University, Tehran-Qom Express Way, Tehran, 3319118651, Iran.
Molecular Microbiology Research Center and Department of Biology, Shahed University, Tehran, Iran.
Sci Rep. 2022 Jul 22;12(1):12576. doi: 10.1038/s41598-022-16894-w.
Acinetobacter baumannii easily turns into pan drug-resistant (PDR) with a high mortality rate. No effective commercial antibiotic or approved vaccine is available against drug-resistant strains of this pathogen. Egg yolk immunoglobulin (IgY) could be used as a simple and low-cost biotherapeutic against its infections. This study evaluates the prophylactic potential of IgY against A. baumannii in a murine pneumonia model. White Leghorn hens were immunized with intramuscular injection of the recombinant biofilm-associated protein (Bap) from A. baumannii on days 0, 21, 42, and 63. The reactivity and antibiofilm activity of specific IgYs raised against the Bap was evaluated by indirect ELISA and a microtiter plate assay for biofilm formation. The IgYs against Bap were able to decrease the biofilm formation ability of A. baumannii and protect the mice against the challenge of A. baumannii. IgYs antibody raised here shows a good antigen-specificity and protectivity which can be used in passive immunotherapy against A. baumannii. In conclusion, the IgY against biofilm-associated protein proves prophylactic in a murine pneumonia model.
鲍曼不动杆菌很容易变成泛耐药（PDR），死亡率很高。针对这种病原体的耐药菌株，尚无有效的商业抗生素或批准的疫苗。卵黄免疫球蛋白（IgY）可用作针对其感染的简单且低成本的生物疗法。本研究在鼠肺炎模型中评估了 IgY 对鲍曼不动杆菌的预防潜力。白来航鸡通过肌肉内注射鲍曼不动杆菌的重组生物膜相关蛋白（Bap）在第 0、21、42 和 63 天进行免疫。通过间接 ELISA 和微滴定板测定生物膜形成来评估针对 Bap 产生的特异性 IgY 的反应性和抗生物膜活性。针对 Bap 的 IgY 能够降低鲍曼不动杆菌的生物膜形成能力，并保护小鼠免受鲍曼不动杆菌的挑战。这里产生的 IgY 抗体显示出良好的抗原特异性和保护作用，可用于针对鲍曼不动杆菌的被动免疫疗法。总之，针对生物膜相关蛋白的 IgY 在鼠肺炎模型中具有预防作用。
