Collage of Dentistry, Umm Al Qura University.
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Cell Mol Biol (Noisy-le-grand). 2022 Feb 28;68(2):19-25. doi: 10.14715/cmb/2022.68.2.3.
The aetiology of oral lichen planus (OLP) is multifactorial, having variable triggers. A role for vitamin D related to the immune system has been established. Vitamin D modulating effect is on the adaptive and innate immune responses. Our study aimed to compare serum levels of vitamin D in patients having different clinical symptoms of OLP (symptomatic or asymptomatic) with healthy individuals. Also, in this study, for further evaluation, the expression level of interleukin-17A and interleukin-6 (IL-17A and IL-6) was evaluated because the presence of active vitamin D reduces the expression of these pro-inflammatory factors. This study was included three groups with 30 volunteers in each. The first group included asymptomatic oral lichen planus patients (reticular or plaque-like lesions). The second group consisted of symptomatic oral lichen planus patients (atrophic or bullous-erosive lesions). In contrast, the third group consisted of healthy control subjects. The serum 25-hydroxyvitamin D was measured between the three groups and then correlated with clinical manifestation of oral lichen planus, either symptomatic or non-symptomatic. The Real-Time PCR technique was used to evaluate the expression of IL-17A and IL-6. Patients with symptomatic OLP (second group) had statistically significantly lower Vitamin D levels than asymptomatic OLP patients (first group). Healthy Controls (third group) exhibited statistically significantly higher vitamin D levels than OLP groups. The results of IL-17A and IL-6 genes expression showed that the presence of vitamin D had a statistically significant effect on reducing the expression of these two pro-inflammatory cytokines among symptomatic and asymptomatic OLP patients. Also, the results showed that there was a statistically significant difference between OLP patients (group I and II) and the control group (group III). In general, the current study results showed that lack of vitamin D had an important role in initiating or increasing the OLP's severity.
口腔扁平苔藓（OLP）的病因是多因素的，具有不同的诱因。已经确定维生素 D 与免疫系统有关。维生素 D 的调节作用在于适应性和先天免疫反应。我们的研究旨在比较具有不同临床症状（有症状或无症状）的 OLP 患者与健康个体的血清维生素 D 水平。此外，在这项研究中，为了进一步评估，评估了白细胞介素-17A 和白细胞介素-6（IL-17A 和 IL-6）的表达水平，因为活性维生素 D 的存在会降低这些促炎因子的表达。
这项研究包括三组，每组 30 名志愿者。第一组包括无症状口腔扁平苔藓患者（网状或斑块状病变）。第二组由有症状口腔扁平苔藓患者（萎缩或大疱性糜烂性病变）组成。相比之下，第三组由健康对照组组成。在三组之间测量血清 25-羟维生素 D，然后将其与口腔扁平苔藓的临床表现（有症状或无症状）相关联。使用实时 PCR 技术评估 IL-17A 和 IL-6 的表达。有症状 OLP 患者（第二组）的维生素 D 水平明显低于无症状 OLP 患者（第一组）。健康对照组（第三组）的维生素 D 水平明显高于 OLP 组。IL-17A 和 IL-6 基因表达的结果表明，维生素 D 的存在对减少这两种促炎细胞因子在有症状和无症状 OLP 患者中的表达有统计学显著影响。此外，结果表明 OLP 患者（第 I 组和第 II 组）和对照组（第 III 组）之间存在统计学显著差异。总的来说，目前的研究结果表明，维生素 D 缺乏在引发或增加 OLP 的严重程度方面起着重要作用。
