Key Laboratory of Horticultural Plant Biology of MOE (Ministry of Education), Huazhong Agricultural University, Wuhan, China.
Key Laboratory of Horticultural Plant Biology of MOE (Ministry of Education), Huazhong Agricultural University, Wuhan, China.
Methods Enzymol. 2022;670:179-212. doi: 10.1016/bs.mie.2022.01.006. Epub 2022 Feb 1.
Citrus is an important horticultural crop with global, economic and nutritional value. Carotenoids represent the main pigments in citrus fruits and contribute to the esthetic and nutritional value. The complexity of carotenoids in citrus poses a challenge for carotenoid analysis. In this chapter, we describe methods for carotenoid extraction, detection, and analysis that have been optimized for study of citrus fruits. High-performance liquid chromatography (HPLC) and Ultrahigh-performance liquid chromatography-high-resolution tandem mass spectrometry (UHPLC-HRMS/MS) strategies are used for carotenoid profiling of citrus fruit carotenoids and are explained in detail. We outline the applications, advantages, and disadvantages of using these methods to analyze carotenoids in the main citrus species including mandarin, orange, and pummelo.
柑橘是一种具有全球经济和营养价值的重要园艺作物。类胡萝卜素是柑橘类水果的主要色素，对其外观和营养价值有重要影响。柑橘类果实中类胡萝卜素的复杂性给类胡萝卜素分析带来了挑战。本章描述了优化后的柑橘果实类胡萝卜素提取、检测和分析方法。高效液相色谱（HPLC）和超高效液相色谱-高分辨率串联质谱（UHPLC-HRMS/MS）策略用于柑橘果实类胡萝卜素的 profiling 分析，并进行了详细说明。我们概述了这些方法在分析主要柑橘品种（包括橘、橙和柚）类胡萝卜素中的应用、优点和缺点。
