Department of Molecular Medicine, UF Scripps Biomedical Research, 130 Scripps Way, Jupiter 33458, FL, USA.
Department of Molecular Medicine, UF Scripps Biomedical Research, 130 Scripps Way, Jupiter 33458, FL, USA.
J Mol Biol. 2023 Jan 15;435(1):167757. doi: 10.1016/j.jmb.2022.167757. Epub 2022 Jul 21.
Signal transduction at the synapse is mediated by a variety of protein-lipid interactions, which are vital for the spatial and temporal regulation of synaptic vesicle biogenesis, neurotransmitter release, and postsynaptic receptor activation. Therefore, our understanding of synaptic transmission cannot be completed until the elucidation of these critical protein-lipid interactions. On this front, recent advances in nanodiscs have vastly expanded our ability to probe and reprogram membrane biology in synapses. Here, we summarize the progress of the nanodisc toolbox and discuss future directions in this exciting field.
在突触处的信号转导是由多种蛋白-脂质相互作用介导的，这些相互作用对于突触小泡生物发生、神经递质释放和突触后受体激活的时空调节至关重要。因此，在阐明这些关键的蛋白-脂质相互作用之前，我们无法完全理解突触传递。在这一方面，纳米盘的最新进展极大地扩展了我们在突触中探测和重新编程膜生物学的能力。在这里，我们总结了纳米盘工具包的进展，并讨论了这个令人兴奋的领域的未来方向。
