Gouka Linda, Vogels Caroline, Hansen Lars H, Raaijmakers Jos M, Cordovez Viviane
Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, Netherlands.
Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
Front Plant Sci. 2022 Jul 7;13:908628. doi: 10.3389/fpls.2022.908628. eCollection 2022.
The phyllosphere, the aboveground part of a plant, is a harsh environment with diverse abiotic and biotic stresses, including oscillating nutrient availability and temperature as well as exposure to UV radiation. Microbial colonization of this dynamic environment requires specific adaptive traits, including tolerance to fluctuating temperatures, the production of secondary metabolites and pigments to successfully compete with other microorganisms and to withstand abiotic stresses. Here, we isolated 175 yeasts, comprising 15 different genera, from the wheat flag leaf and characterized a selection of these for various adaptive traits such as substrate utilization, tolerance to different temperatures, biofilm formation, and antagonism toward the fungal leaf pathogen . Collectively our results revealed that the wheat flag leaf is a rich resource of taxonomically and phenotypically diverse yeast genera that exhibit various traits that can contribute to survival in the harsh phyllosphere environment.
植物的叶际，即植物的地上部分，是一个充满各种非生物和生物胁迫的恶劣环境，包括营养物质供应和温度的波动以及紫外线辐射。微生物在这个动态环境中的定殖需要特定的适应性状，包括对温度波动的耐受性、产生次生代谢产物和色素以成功与其他微生物竞争并抵御非生物胁迫。在这里，我们从小麦旗叶中分离出175株酵母，它们分属于15个不同的属，并对其中一部分酵母的各种适应性状进行了表征，如底物利用、对不同温度的耐受性、生物膜形成以及对真菌叶部病原体的拮抗作用。我们的研究结果共同表明，小麦旗叶是分类学和表型多样的酵母属的丰富资源，这些酵母表现出有助于在恶劣叶际环境中生存的各种性状。
