Liu An-An, Sun En-Ze, Wang Zhi-Gang, Liu Shu-Lin, Pang Dai-Wen
State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Frontiers Science Center for New Organic Matter, Research Center for Analytical Sciences, College of Chemistry, School of Medicine, Frontiers Science Center for Cell Responses, Nankai University, Tianjin 300071, China.
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Natl Sci Rev. 2021 Sep 9;9(6):nwab162. doi: 10.1093/nsr/nwab162. eCollection 2022 Jun.
Live cells, as reservoirs of biochemical reactions, can serve as amazing integrated chemical plants where precursor formation, nucleation and growth of nanocrystals, and functional assembly, can be carried out accurately following an artificial program. It is crucial but challenging to deliberately direct intracellular pathways to synthesize desired nanocrystals that cannot be produced naturally in cells, because the relevant reactions exist in different spatiotemporal dimensions and will never encounter each other spontaneously. This article summarizes the progress in the introduction of inorganic functional nanocrystals into live cells via the 'artificially regulated space-time-coupled live-cell synthesis' strategy. We also describe ingenious bio-applications of nanocrystal-cell systems, and quasi-biosynthesis strategies expanded from live-cell synthesis. Artificially regulated live-cell synthesis-which involves the interdisciplinary application of biology, chemistry, nanoscience and medicine-will enable researchers to better exploit the unanticipated potentialities of live cells and open up new directions in synthetic biology.
活细胞作为生化反应的储存库，可充当令人惊叹的集成化工厂，在其中可以按照人工程序精确地进行前体形成、纳米晶体的成核与生长以及功能组装。刻意引导细胞内途径以合成细胞中无法自然产生的所需纳米晶体至关重要但具有挑战性，因为相关反应存在于不同的时空维度，且永远不会自发相遇。本文总结了通过“人工调控时空耦合活细胞合成”策略将无机功能纳米晶体引入活细胞方面的进展。我们还描述了纳米晶体 - 细胞系统巧妙的生物应用，以及从活细胞合成扩展而来的准生物合成策略。人工调控的活细胞合成——涉及生物学、化学、纳米科学和医学的跨学科应用——将使研究人员能够更好地挖掘活细胞未被预料到的潜力，并为合成生物学开辟新方向。
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