Department of Neurosurgery, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan 250014, P.R. China.
Medical Science and Technology Innovation Center, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan 250117, P.R. China.
Nano Lett. 2022 Aug 10;22(15):6391-6401. doi: 10.1021/acs.nanolett.2c02259. Epub 2022 Jul 25.
Exosomes derived from mesenchymal stem cells (MSCs) have been proven to exhibit great potentials in spinal cord injury (SCI) therapy. However, conventional two-dimensional (2D) culture will inevitably lead to the loss of stemness of MSCs, which substantially limits the therapeutic potency of MSCs exosomes (2D-Exo). Exosomes derived from three-dimensional culture (3D-Exo) possess higher therapeutic efficiency which have wide applications in spinal cord therapy. Typically, conventional exosome therapy that relies on local repeated injection results in secondary injury and low efficiency. It is urgent to develop a more reliable, convenient, and effective exosome delivery method to achieve constant exosomes release. Herein, we proposed a controlled 3D-exohydrogel hybrid microneedle array patch to achieve SCI repair . Our studies suggested that MSCs with 3D-culturing could maintain their stemness, and consequently, 3D-Exo effectively reduced SCI-induced inflammation and glial scarring. Thus, it is a promising therapeutic strategy for the treatment of SCI.
间充质干细胞（MSCs）来源的外泌体已被证明在脊髓损伤（SCI）治疗中具有巨大的潜力。然而，传统的二维（2D）培养方法不可避免地会导致MSCs 干性的丧失，这极大地限制了 MSCs 外泌体（2D-Exo）的治疗效力。三维培养（3D-Exo）衍生的外泌体具有更高的治疗效率，在脊髓治疗中有广泛的应用。通常，依赖于局部重复注射的传统外泌体治疗会导致二次损伤和低效率。因此，迫切需要开发一种更可靠、方便、有效的外泌体递送方法，以实现持续的外泌体释放。在这里，我们提出了一种受控的 3D-Exo 水凝胶混合微针贴片，以实现 SCI 修复。我们的研究表明，进行 3D 培养的 MSCs 可以保持其干性，并且 3D-Exo 可以有效减轻 SCI 诱导的炎症和神经胶质瘢痕形成。因此，这是一种有前途的 SCI 治疗策略。
