Sari Mona, Ana Ika Dewi, Yusuf Yusril
Department of Physics, Faculty of Mathematics and Natural Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Department of Dental Biomedical Sciences, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Bioengineering (Basel). 2022 Jul 18;9(7):325. doi: 10.3390/bioengineering9070325.
In this work, carbonated hydroxyapatite/titanium alloy (CHA/Ti) and carbonated hydroxyapatite/honeycomb/titanium alloy (CHA/HCB/Ti) plates were coated using the electrophoretic deposition dip coating (EP2D) method. Analysis of cell viability and surface morphology of CHA/Ti and CHA/HCB/Ti coatings were carried out using the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and scanning electron microscopy (SEM), respectively. In a previous study, the thickness and average compressive strength values for the CHA/Ti and CHA/HCB/Ti plates were about 63−89 μm and 54−75 MPa, respectively. The result for thickness and compressive strength in this research followed the thickness and compressive strength parameters for coating in bone implants. In this work, the cell viability for incubation times during 24 h and 48 h of CHA/Ti plates is demonstrably superior to that of CHA/HCB/Ti plates, respectively, where the cell viability for CHA/Ti plates increased to ((67 ± 2)%) after incubation for 48 h. According to the one-way analysis of variance (ANOVA), the p-value was <0.05, indicating a significant difference in the average cell viability value across the three groups. Furthermore, the surface of CHA/Ti is not changed after the coating process. These results will yield many positive biomedical applications, especially in bone implants. Overall, CHA/Ti and CHA/HCB/Ti plates can be considered candidates for biomedical applications based on an analysis of surface morphology and cell viability.
在本研究中，采用电泳沉积浸涂法（EP2D）制备了碳酸化羟基磷灰石/钛合金（CHA/Ti）板和碳酸化羟基磷灰石/蜂窝/钛合金（CHA/HCB/Ti）板。分别使用MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）法和扫描电子显微镜（SEM）对CHA/Ti和CHA/HCB/Ti涂层的细胞活力和表面形态进行了分析。在先前的一项研究中，CHA/Ti板和CHA/HCB/Ti板的厚度和平均抗压强度值分别约为63−89μm和54−75MPa。本研究中涂层的厚度和抗压强度结果符合骨植入物涂层的厚度和抗压强度参数。在本研究中，CHA/Ti板在24小时和48小时孵育时间的细胞活力分别明显优于CHA/HCB/Ti板，其中CHA/Ti板在孵育48小时后细胞活力增加到（(67±2)%）。根据单因素方差分析（ANOVA），p值<0.05，表明三组的平均细胞活力值存在显著差异。此外，CHA/Ti的表面在涂层过程后没有变化。这些结果将产生许多积极的生物医学应用，特别是在骨植入物方面。总体而言，基于表面形态和细胞活力分析，CHA/Ti板和CHA/HCB/Ti板可被视为生物医学应用的候选材料。
