Grochowska Marta, Laskus Tomasz, Paciorek Marcin, Pollak Agnieszka, Lechowicz Urszula, Makowiecki Michał, Horban Andrzej, Radkowski Marek, Perlejewski Karol
Department of Immunopathology of Infectious and Parasitic Diseases, Medical University of Warsaw, 02-106 Warsaw, Poland.
Department of Adult Infectious Diseases, Medical University of Warsaw, 01-201 Warsaw, Poland.
Curr Issues Mol Biol. 2022 Jun 29;44(7):2903-2914. doi: 10.3390/cimb44070200.
There are multiple lines of evidence for the existence of communication between the central nervous system (CNS), gut, and intestinal microbiome. Despite extensive analysis conducted on various neurological disorders, the gut microbiome was not yet analyzed in neuroinfections. In the current study, we analyzed the gut microbiome in 47 consecutive patients hospitalized with neuroinfection (26 patients had viral encephalitis/meningitis; 8 patients had bacterial meningitis) and in 20 matched for age and gender health controls. Using the QIIME pipeline, 16S rRNA sequencing and classification into operational taxonomic units (OTUs) were performed on the earliest stool sample available. Bacterial taxa such as , , , , and were decreased in patients with neuroinfection when compared to controls. Alpha diversity metrics showed lower within-sample diversity in patients with neuroinfections, though there were no differences in beta diversity. Furthermore, there was no significant change by short-term (1-3 days) antibiotic treatment on the gut microbiota, although alpha diversity metrics, such as Chao1 and Shannon's index, were close to being statistically significant. The cause of differences between patients with neuroinfections and controls is unclear and could be due to inflammation accompanying the disease; however, the effect of diet modification and/or hospitalization cannot be excluded.
有多项证据表明中枢神经系统（CNS）、肠道和肠道微生物群之间存在通讯。尽管对各种神经系统疾病进行了广泛分析，但尚未对神经感染中的肠道微生物群进行分析。在本研究中，我们分析了47例连续住院的神经感染患者（26例患有病毒性脑炎/脑膜炎；8例患有细菌性脑膜炎）以及20名年龄和性别匹配的健康对照者的肠道微生物群。使用QIIME流程，对可得的最早粪便样本进行16S rRNA测序并分类为操作分类单元（OTU）。与对照组相比，神经感染患者中的某些细菌分类群如 、 、 、 和 减少。α多样性指标显示神经感染患者的样本内多样性较低，尽管β多样性没有差异。此外，短期（1 - 3天）抗生素治疗对肠道微生物群没有显著变化，尽管Chao1和香农指数等α多样性指标接近具有统计学意义。神经感染患者与对照组之间差异的原因尚不清楚，可能是由于疾病伴随的炎症；然而，饮食改变和/或住院的影响也不能排除。
