Minkiewicz Piotr, Mattison Christopher P, Darewicz Małgorzata
Chair of Food Biochemistry, University of Warmia and Mazury in Olsztyn, Plac Cieszyński 1, 10-726 Olsztyn-Kortowo, Poland.
Southern Regional Research Center, FPSQ, ARS, U.S. Department of Agriculture, New Orleans, LA 70124, USA.
Curr Issues Mol Biol. 2022 Jul 6;44(7):3100-3117. doi: 10.3390/cimb44070214.
The aim of the study presented here was to determine if there is a correlation between the presence of specific protein domains within tree nut allergens or tree nut allergen epitopes and the frequency of bioactive fragments and the predicted susceptibility to enzymatic digestion in allergenic proteins from tree nuts of cashew (), pecan (), English walnut () and pistachio () plants. These bioactive peptides are distributed along the length of the protein and are not enriched in IgE epitope sequences. Classification of proteins as bioactive peptide precursors based on the presence of specific protein domains may be a promising approach. Proteins possessing a vicilin, N-terminal family domain, or napin domain contain a relatively low occurrence of bioactive fragments. In contrast, proteins possessing the cupin 1 domain without the vicilin N-terminal family domain contain a relatively high total frequency of bioactive fragments and predicted release of bioactive fragments by the joint action of pepsin, trypsin, and chymotrypsin. This approach could be utilized in food science to simplify the selection of protein domains enriched for bioactive peptides.
本文所呈现的研究目的是确定腰果（）、山核桃（）、英国胡桃（）和阿月浑子（）植物坚果中的变应原蛋白内特定蛋白结构域或坚果变应原表位的存在，与生物活性片段的频率以及变应原蛋白中酶促消化的预测易感性之间是否存在相关性。这些生物活性肽沿蛋白质长度分布，且在IgE表位序列中未富集。基于特定蛋白结构域的存在将蛋白质分类为生物活性肽前体可能是一种有前景的方法。具有豌豆球蛋白、N端家族结构域或napin结构域的蛋白质含有相对较少的生物活性片段。相比之下，具有无豌豆球蛋白N端家族结构域的cupin 1结构域的蛋白质含有相对较高的生物活性片段总频率，并且通过胃蛋白酶、胰蛋白酶和胰凝乳蛋白酶的联合作用预测生物活性片段会释放。这种方法可用于食品科学，以简化对富含生物活性肽的蛋白质结构域的选择。
