Kukla Sergey Petrovich, Chelomin Victor Pavlovich, Mazur Andrey Alexandrovich, Slobodskova Valentina Vladimirovna
Il'ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, Russia.
Toxics. 2022 Jun 24;10(7):348. doi: 10.3390/toxics10070348.
Products containing nanomaterials are becoming more and more common in everyday life. Zinc oxide nanoparticles (ZnO NPs), meanwhile, are among the most widely used NPs. However, their genotoxic effect on the germ products of marine organisms is poorly understood. Therefore, the effects of ZnO NPs and zinc ions (20, 50, 100, 200 µg/L) on the sperm of sand dollar were compared. Comet assay showed that both tested pollutants caused an increase in DNA damage to 6.57 ± 2.41 and 7.42 ± 0.88% DNA in the comet tail, for zinc ions and ZnO NPs, respectively. Additionally, a different pattern was shown by the increase in DNA damage, with increasing concentration of pollutants, in different experimental groups.
含有纳米材料的产品在日常生活中越来越常见。与此同时，氧化锌纳米颗粒（ZnO NPs）是使用最为广泛的纳米颗粒之一。然而，它们对海洋生物生殖产物的遗传毒性作用却鲜为人知。因此，比较了ZnO NPs和锌离子（20、50、100、200 µg/L）对海胆精子的影响。彗星试验表明，两种受试污染物都会导致DNA损伤增加，锌离子和ZnO NPs分别使彗星尾中的DNA损伤增加至6.57±2.41%和7.42±0.88%。此外，不同实验组中，随着污染物浓度增加，DNA损伤增加呈现出不同的模式。
