Technology Innovation Research Division, World Institute of Kimchi, Gwangju 61755, Republic of Korea.
Division of Applied Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Republic of Korea.
J Microbiol Biotechnol. 2022 Aug 28;32(8):960-966. doi: 10.4014/jmb.2205.05019. Epub 2022 Jul 28.
Lactic acid bacteria (LAB) exert antagonistic activity against root-knot nematodes, mainly by producing organic acids via carbohydrate fermentation. However, they have not yet been used for root-knot nematode () control owing to a lack of economic feasibility and effectiveness. In this study, we aimed to isolate organic acid-producing LAB from kimchi (Korean traditional fermented cabbage) and evaluated their nematicidal activity. Among the 234 strains isolated, those showing the highest nematicidal activity were selected and identified as WiKim0090. Nematicidal activity and egg hatch inhibitory activity of WiKim0090 culture filtrate were dose dependent. Nematode mortality 3 days after treatment with 2.5% of the culture filtrate was 100%, with a 50% lethal concentration of 1.41%. In pot tests, the inhibitory activity of an WiKim0090-copper sulfate mixture on gall formation increased. Compared to abamectin application, which is a commercial nematicide, a higher control value was observed using the WiKim0090-copper sulfate mixture, indicating that this combination can be effective in controlling the root-knot nematode.
乳酸菌 (LAB) 通过碳水化合物发酵产生有机酸，从而对根结线虫发挥拮抗活性。然而，由于缺乏经济性和有效性，它们尚未被用于根结线虫的防治。在这项研究中，我们旨在从泡菜（韩国传统发酵白菜）中分离产有机酸的 LAB，并评估其杀线虫活性。在分离出的 234 株菌中，选择具有最高杀线虫活性的菌株并鉴定为 WiKim0090。WiKim0090 培养滤液的杀线虫活性和卵孵化抑制活性呈剂量依赖性。用 2.5%培养滤液处理 3 天后，线虫死亡率为 100%，半数致死浓度为 1.41%。在盆栽试验中，WiKim0090-硫酸铜混合物对根结形成的抑制活性增加。与商业杀线虫剂阿维菌素的应用相比，使用 WiKim0090-硫酸铜混合物观察到更高的防治值，表明这种组合可有效防治根结线虫。
