Lee Yong Seong, Naning Kyaw Wai, Nguyen Xuan Hoa, Kim Sun Bae, Moon Jae Hak, Kim Kil Yong
Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture, Chonnam National University, Gwangju 500-757, Republic of Korea.
J Microbiol Biotechnol. 2014 Nov 28;24(11):1510-5. doi: 10.4014/jmb.1405.05014.
Lysobacter capsici YS1215 isolated from soil previously showed nematicidal potential for biological control of the root-knot nematode. In this study, lactic acid, a nematicidal compound, was isolated from culture filtrate of YS1215, and its ovicidal activity was investigated. Purification and identification of lactic acid were performed by a series of column chromatographies and identified by (1)H and (13)C NMR spectra and GC-MS analysis. Our results showed that bacterial culture filtrate containing lactic acid significantly inhibited egg hatching. The lowest egg hatch rate (5.9%) was found at a high concentration (25 μl/ml) of lactic acid at 5 days after incubation, followed by 20 (15.2%), 15 (23.7%), 10 (29.8%), and 5 (36.4%) μl/ml, while egg hatching in the control (sterile distilled water) was 44.5%. This is the first report of lactic acid as an ovicidal compound, and it may be considered as an alternative of chemical pesticide against root-knot nematodes.
先前从土壤中分离出的辣椒溶杆菌YS1215对根结线虫的生物防治显示出杀线虫潜力。在本研究中，从YS1215的培养滤液中分离出一种杀线虫化合物乳酸，并对其杀卵活性进行了研究。通过一系列柱色谱法对乳酸进行纯化和鉴定，并通过¹H和¹³C NMR光谱以及GC-MS分析进行鉴定。我们的结果表明，含有乳酸的细菌培养滤液显著抑制卵孵化。在孵化5天后，乳酸高浓度（25 μl/ml）时卵孵化率最低（5.9%），其次是20（15.2%）、15（23.7%）、10（29.8%）和5（36.4%）μl/ml，而对照（无菌蒸馏水）中的卵孵化率为44.5%。这是关于乳酸作为杀卵化合物的首次报道，它可能被视为对抗根结线虫的化学农药替代品。
