Yan Zhiqiang, Deng Ruyue, Zhang Hongwei, Li Jiali, Zhu Susong
Guizhou Academy of Agricultural Sciences, Guiyang, 550000 China.
3 Biotech. 2022 Sep;12(9):188. doi: 10.1007/s13205-022-03226-y. Epub 2022 Jul 22.
Floret opening and closure are critical for rice to complete reproductive development. To further understand the molecular mechanism of floret opening and closure in rice, RNA-seq was performed on the floret of Indica and Japonica rice in the state of 1-h before floret opening, at the opening and closure, respectively. Our results show that many differentially expressed genes are produced throughout the floret opening and closure of both Indica and Japonica rice. Differentially expressed genes shared between Indica and Japonica rice at floret opening were involved in seven metabolic pathways, including plant hormone signal transduction, MAPK signaling pathway-plant, starch and sucrose metabolism, alpha-Linolenic acid metabolism, plant-pathogen interaction, diterpenoid biosynthesis, glucuronate interconversions, phenylpropanoid biosynthesis, compared to 1 h before floret opening. In addition, the expression patterns of some genes, , and which the above metabolic pathways, were different between Indica and Japonica rice. Compared to the floret opening, the differentially expressed genes at floret closure were mainly involved in the following three metabolic pathways: Circadian rhythm-plant, sesquiterpenoid and triterpenoid biosynthesis, starch and sucrose metabolism, and thiamine metabolism. This study provides insights into revealing the molecular mechanism of floret opening and closure in rice.
The online version contains supplementary material available at 10.1007/s13205-022-03226-y.
小花的开闭对水稻完成生殖发育至关重要。为了进一步了解水稻小花开闭的分子机制，分别在籼稻和粳稻小花开放前1小时、开放时和关闭时对其小花进行了RNA测序。我们的结果表明，在籼稻和粳稻小花的整个开闭过程中都产生了许多差异表达基因。与小花开放前1小时相比，籼稻和粳稻在小花开放时共有的差异表达基因涉及七种代谢途径，包括植物激素信号转导、植物中的MAPK信号通路、淀粉和蔗糖代谢、α-亚麻酸代谢、植物-病原体相互作用、二萜生物合成、葡萄糖醛酸相互转化、苯丙烷生物合成。此外，上述代谢途径中的一些基因，如 、 和 ，在籼稻和粳稻中的表达模式不同。与小花开放相比，小花关闭时的差异表达基因主要涉及以下三种代谢途径：植物昼夜节律、倍半萜和三萜生物合成、淀粉和蔗糖代谢以及硫胺素代谢。本研究为揭示水稻小花开闭的分子机制提供了见解。
在线版本包含可在10.1007/s13205-022-03226-y获取的补充材料。
