使用聚焦紧密的飞秒激光来光活化脂肪来源的干细胞分化的方案。
Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser.
机构信息
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, P.R. China.
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, P.R. China.
出版信息
STAR Protoc. 2022 Jul 19;3(3):101574. doi: 10.1016/j.xpro.2022.101574. eCollection 2022 Sep 16.
The technology to induce stem cell differentiation is of great importance in life science, stem cell therapy, and regenerative medicine. Here, we detail steps to noninvasively activate stem cell differentiation and using a tightly focused femtosecond laser. We describe how a single-time transient photoactivation can initiate differentiation without any gene engineering, exogenous substances, or physical contact. This protocol enables the differentiation of adipose-derived stem cells to osteoblasts and cerebellar granule neuron progenitors to granule neurons . For complete details on the use and execution of this protocol, please refer to Tang et al. (2022).
在生命科学、干细胞疗法和再生医学中，诱导干细胞分化的技术非常重要。在这里，我们详细介绍了如何使用聚焦紧密的飞秒激光进行非侵入性激活干细胞分化的步骤。我们描述了单次瞬时光激活如何在不进行任何基因工程、外源物质或物理接触的情况下启动分化。该方案可使脂肪来源的干细胞向成骨细胞分化，小脑颗粒神经元前体细胞向颗粒神经元分化。如需详细了解该方案的使用和实施，请参考 Tang 等人（2022 年）的研究。
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