Westley B R, May F E
Nucleic Acids Res. 1987 May 11;15(9):3773-86. doi: 10.1093/nar/15.9.3773.
A cDNA library was constructed from the mRNA of the ZR-75 oestrogen responsive human breast cancer cell line and screened for oestrogen regulated mRNA sequences. Of the recombinants isolated, 30 contained cDNA that corresponded to a single mRNA species of 2.1 kb that was induced between 10 and 15 fold by oestradiol treatment. The sequence of the entire open reading frame and 3' non-coding region of the mRNA was determined and shown to encode the aspartyl protease cathepsin D. The induction of cathepsin D mRNA is specific for oestrogen and is maximal at 3 X 10(-10) M. Cathepsin D mRNA levels were increased by oestrogen in 3 oestrogen responsive breast cancer cell lines. Cathepsin D mRNA was expressed but not regulated in an oestrogen receptor negative breast tumour cell line, BT 20, and in 2 other malignant cell lines, Hela and A431.
从ZR - 75雌激素反应性人乳腺癌细胞系的mRNA构建了一个cDNA文库，并筛选雌激素调节的mRNA序列。在分离出的重组体中，30个含有与一种2.1 kb的单一mRNA种类相对应的cDNA，该mRNA在雌二醇处理后诱导增加了10至15倍。测定了该mRNA的整个开放阅读框和3'非编码区的序列，并显示其编码天冬氨酸蛋白酶组织蛋白酶D。组织蛋白酶D mRNA的诱导对雌激素具有特异性，在3×10(-10)M时达到最大值。在3种雌激素反应性乳腺癌细胞系中，雌激素增加了组织蛋白酶D mRNA水平。在雌激素受体阴性的乳腺肿瘤细胞系BT 20以及另外两种恶性细胞系Hela和A431中，组织蛋白酶D mRNA有表达但不受调节。
