Winstanley J H, Leinster S J, Cooke T G, Westley B R, Platt-Higgins A M, Rudland P S
Department of Surgery, University of Liverpool Medical School, UK.
Br J Cancer. 1993 Apr;67(4):767-72. doi: 10.1038/bjc.1993.139.
The expression of the protease cathepsin-D has been evaluated using an immunohistochemical technique with a polyclonal antibody in paraffin-embedded tissue from 359 patients treated between the years 1975-1981 for Stage I and II breast cancer. One hundred and twenty seven patients (35%) have strongly positive, granular staining, 138 (38%) are intermediately stained in the cytoplasm, and in 94 (26%) no staining is observed. There is a strong positive association between expression of cathepsin-D and the presence of tumour in axillary lymph nodes (P < 0.006). Expression of the protease is associated with significantly poorer survival of patients in univariate analysis (P = 0.025); however, this is not independent of other tumour variables.
采用免疫组化技术,使用多克隆抗体对1975年至1981年间接受治疗的359例I期和II期乳腺癌患者石蜡包埋组织中的蛋白酶组织蛋白酶-D表达进行了评估。127例患者(35%)有强阳性颗粒状染色,138例(38%)细胞质呈中度染色,94例(26%)未观察到染色。组织蛋白酶-D的表达与腋窝淋巴结肿瘤的存在之间存在强正相关(P < 0.006)。在单因素分析中,该蛋白酶的表达与患者生存率显著较低相关(P = 0.025);然而,这并非独立于其他肿瘤变量。