Galgano Michela, Capozza Paolo, Pellegrini Francesco, Cordisco Marco, Sposato Alessio, Sblano Sabina, Camero Michele, Lanave Gianvito, Fracchiolla Giuseppe, Corrente Marialaura, Cirone Francesco, Trotta Adriana, Tempesta Maria, Buonavoglia Domenico, Pratelli Annamaria
Department of Veterinary Medicine, University Aldo Moro of Bari, 70010 Valenzano, Italy.
Department of Pharmacy-Drug Sciences, University Aldo Moro of Bari, 70125 Bari, Italy.
Antibiotics (Basel). 2022 Jul 20;11(7):979. doi: 10.3390/antibiotics11070979.
The spread of extended-spectrum β-lactamase-producing and methicillin-resistant has caused a reduction in antibiotic effectiveness and an increase in mortality rates. Essential oils (EOs), known for their therapeutic efficacy, can be configured as novel broad-spectrum biocides. Accordingly, the bacteriostatic-bactericidal activity of (LEO), (PEO), (FEO), (BEO), (MEO), (TEO), and (GEO), at concentrations ranging from 1.25 to 40% (/), were tested in vitro against different and strains using minimal inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). The chemical compositions of the EOs were analyzed using GC/MS. The major components of all seven tested oils were limonene, α-pinene, anethole, estragole, citral, thymol, and zingiberene, respectively. We found that the bacteriostatic-bactericidal activity of the EOs was related to their chemotypes and concentrations, as well as the strain of the bacteria. A dose-effect correlation was found when testing GEO against strains whilst FEO was found to have no activity regardless of concentration. PEO, MEO, and BEO were found to have bactericidal effect with a MIC and MBC of 1.25% (/) against strains, and LEO was found to have values of 1.25% (/) and 5% (/) against ATCC and clinical isolate, respectively. Interestingly, the antimicrobial activity of TEO was not related to oil concentration and the complete inhibition of growth across all and was observed. Although preliminary, our data demonstrate the efficacy of EOs and pave the way for further investigations on their potential synergistic use with traditional drugs in the human and veterinary fields.
产超广谱β-内酰胺酶和耐甲氧西林细菌的传播导致抗生素效力降低和死亡率上升。精油（EOs）以其治疗功效而闻名，可被配置为新型广谱杀菌剂。因此，使用最低抑菌浓度（MICs）和最低杀菌浓度（MBCs），在体外测试了柠檬桉叶油（LEO）、薄荷油（PEO）、小茴香油（FEO）、月桂叶油（BEO）、肉豆蔻油（MEO）、百里香油（TEO）和姜油（GEO）在1.25%至40%（体积/体积）浓度范围内对不同革兰氏阳性和革兰氏阴性菌株的抑菌杀菌活性。使用气相色谱/质谱联用仪（GC/MS）分析了精油的化学成分。所有七种测试油的主要成分分别是柠檬烯、α-蒎烯、茴香脑、草蒿脑、柠檬醛、百里香酚和姜烯。我们发现，精油的抑菌杀菌活性与其化学类型、浓度以及细菌菌株有关。在测试GEO对革兰氏阴性菌株时发现了剂量效应相关性，而无论浓度如何，FEO均无活性。发现PEO、MEO和BEO对革兰氏阳性菌株具有杀菌作用，MIC和MBC为1.25%（体积/体积），而LEO对ATCC和临床分离株的值分别为1.25%（体积/体积）和5%（体积/体积）。有趣的是，TEO的抗菌活性与油浓度无关，并且观察到对所有革兰氏阳性和革兰氏阴性菌株的生长均有完全抑制作用。尽管是初步研究，但我们的数据证明了精油的功效，并为进一步研究其在人类和兽医领域与传统药物潜在的协同使用铺平了道路。
