Research Center for Analytical Instrumentation, State Key Laboratory of Industrial Control Technology, College of Control Science and Engineering, Zhejiang University, Hangzhou 310027, China.
College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
Biosensors (Basel). 2022 Jun 24;12(7):450. doi: 10.3390/bios12070450.
Single-cell RNA sequencing (scRNA-seq) technology provides a powerful tool for understanding complex biosystems at the single-cell and single-molecule level. The past decade has been a golden period for the development of single-cell sequencing, with scRNA-seq undergoing a tremendous leap in sensitivity and throughput. The application of droplet- and microwell-based microfluidics in scRNA-seq has contributed greatly to improving sequencing throughput. This review introduces the history of development and important technical factors of scRNA-seq. We mainly focus on the role of microfluidics in facilitating the development of scRNA-seq technology. To end, we discuss the future directions for scRNA-seq.
单细胞 RNA 测序 (scRNA-seq) 技术为在单细胞和单分子水平上理解复杂生物系统提供了强大的工具。过去十年是单细胞测序发展的黄金时期，单细胞 RNA 测序在灵敏度和通量方面取得了巨大飞跃。基于液滴和微井的微流控技术在单细胞 RNA 测序中的应用极大地促进了测序通量的提高。本综述介绍了 scRNA-seq 的发展历史和重要技术因素。我们主要关注微流控在促进 scRNA-seq 技术发展中的作用。最后，我们讨论了 scRNA-seq 的未来方向。
