Thomas Shalom Sara, Cha Youn-Soo, Kim Kyung-Ah
Department of Food Science and Human Nutrition, Jeonbuk National University, Jeonju 54896, Korea.
Obesity Research Center, Jeonbuk National University, Jeonju 54896, Korea.
Foods. 2022 Jul 18;11(14):2124. doi: 10.3390/foods11142124.
Perilla () oil reduces high-fat-diet-induced colon inflammation by suppressing the NF-κB pathway. In the current study, we compared the effect of endogenously produced and externally supplemented omega-3 fatty acids on high-fat-diet-induced colon inflammation. The transgenic mice that endogenously synthesize omega-3 fatty acids were backcrossed with C57BL/6J wild-type mice to obtain transgenic (TR) and wild-type (WT) littermates. Five-week-old male littermates were divided into five groups: two groups fed 10% normal diet (WTLD, TRLD) and three groups fed with a 60% fat high-fat diet (WTHD, TRHD, and WTPO). In the WTPO group, 8% (/) of perilla oil was added. Perilla oil supplemented WT mice and transgenic mice suppressed high-fat-diet-induced body weight and improved serum lipid levels. Furthermore, the WTPO and TRHD groups exhibited increased colon length, lower macroscopic scores, and reduced levels of pro-inflammatory markers and improved epithelial integrity barrier markers. The expression of GPR120 was increased in the WTPO group. Altogether, our results indicated that perilla oil could improve the symptoms of colon inflammation as an alternate omega-3 fatty acid supplement.
紫苏油通过抑制NF-κB信号通路减轻高脂饮食诱导的结肠炎症。在本研究中，我们比较了内源性产生和外源性补充的ω-3脂肪酸对高脂饮食诱导的结肠炎症的影响。将内源性合成ω-3脂肪酸的转基因小鼠与C57BL/6J野生型小鼠回交，以获得转基因（TR）和野生型（WT）同窝小鼠。将5周龄的雄性同窝小鼠分为五组：两组喂食10%的正常饮食（WTLD、TRLD），三组喂食60%脂肪的高脂饮食（WTHD、TRHD和WTPO）。在WTPO组中，添加了8%（/）的紫苏油。补充紫苏油的野生型小鼠和转基因小鼠抑制了高脂饮食诱导的体重增加，并改善了血脂水平。此外，WTPO和TRHD组的结肠长度增加，宏观评分降低，促炎标志物水平降低，上皮完整性屏障标志物得到改善。WTPO组中GPR120的表达增加。总之，我们的结果表明，紫苏油作为一种替代的ω-3脂肪酸补充剂，可以改善结肠炎症症状。
