Key Laboratory of Fruit Breeding Technology of Ministry of Agriculture and Rural Affairs, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
Int J Mol Sci. 2022 Jul 21;23(14):8026. doi: 10.3390/ijms23148026.
Kiwifruit is loved by consumers for its unique taste and rich vitamin C content. Kiwifruit are very sensitive to adverse soil environments owing to fleshy and shallow roots, which limits the uptake of water and nutrients into the root system, resulting in low yield and poor fruit quality. Lateral roots are the key organs for plants to absorb water and nutrients. Improving water and fertilizer use efficiency by promoting lateral root development is a feasible method to improve yield and quality. Expansin proteins plays a major role in lateral root growth; hence, it is important to identify expansin protein family members, screen key genes, and explore gene function in root development. In this study, 41 expansin genes were identified based on the genome of kiwifruit ('Hongyang', ). By clustering with the expansin protein family, the 41 AcExpansin proteins were divided into four subfamilies. The AcExpansin protein family was further analysed by bioinformatics methods and was shown to be evolutionarily diverse and conserved at the DNA and protein levels. Based on previous transcriptome data and quantitative real-time PCR assays, we screened the candidate gene . Overexpression of in kiwifruit increased the number of kiwifruit lateral roots.
猕猴桃因其独特的口感和丰富的维生素 C 含量而深受消费者喜爱。由于肉质浅根，猕猴桃对不良土壤环境非常敏感，这限制了水分和养分被根系吸收，导致产量低、果实品质差。侧根是植物吸收水分和养分的关键器官。通过促进侧根发育来提高水肥利用效率是提高产量和品质的一种可行方法。扩展蛋白在侧根生长中起主要作用；因此，鉴定扩展蛋白家族成员、筛选关键基因并探索基因在根发育中的功能非常重要。在这项研究中，根据猕猴桃（“红阳”）的基因组，鉴定了 41 个扩展蛋白基因。通过与 扩展蛋白家族聚类，将 41 个 AcExpansin 蛋白分为四个亚家族。通过生物信息学方法进一步分析 AcExpansin 蛋白家族，表明其在 DNA 和蛋白质水平上具有多样化和保守的进化。基于先前的转录组数据和定量实时 PCR 分析，我们筛选了候选基因 。在猕猴桃中过表达 增加了猕猴桃侧根的数量。
