Chai Xueying, Ai Zhilu, Liu Jun, Guo Ting, Wu Jingyan, Bai Jie, Lin Qinlu
Key Laboratory of Staple Grain Processing, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Zhengzhou, 450002 Henan China.
National Engineering Laboratory for Deep Process of Rice and Byproducts, Hunan Key Laboratory of Grain-oil Deep Process and Quality Control, Hunan Key Laboratory of Processed Food for Special Medical Purpose, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, 41004 Hunan China.
Food Sci Biotechnol. 2020 Mar 30;29(7):927-937. doi: 10.1007/s10068-020-00745-3. eCollection 2020 Jul.
The effects of the secondary metabolite biosynthesis on the metabolism and morphology of the were investigated in this study. Hypha and septum length became longer after deletion of genes R and CT in LQ-6 by -mediated transformation technology, highly branched hyphae, much smaller and freely dispersed mycelial pellets were observed in . Compared with that in the wild-type, the level of intracellular NADH and NADPH was almost constant in ΔR at 4 days, but the NADH and NADPH levels decreased by 1.58-fold and 3.71-fold in ΔCT. The present study can not only provide a kind of strategy to improve the pigments production, but also provide theoretical support for the further study of relationship between the secondary metabolites, metabolism and morphological change.
本研究考察了次生代谢物生物合成对[具体物种名称未给出]代谢和形态的影响。通过[具体介导技术未给出]介导的转化技术敲除LQ - 6中的基因R和CT后，菌丝和隔膜长度变长，在[具体菌株未给出]中观察到高度分支的菌丝、小得多且自由分散的菌丝球。与野生型相比，ΔR在第4天细胞内NADH和NADPH水平几乎恒定，但ΔCT中的NADH和NADPH水平分别下降了1.58倍和3.71倍。本研究不仅可为提高[具体物种名称未给出]色素产量提供一种策略，还可为进一步研究次生代谢物、代谢和形态变化之间的关系提供理论支持。
