新生儿眼表微生物群的特征与比较
Characterization and Comparison of Ocular Surface Microbiome in Newborns.
作者信息
Petrillo Francesco, Petrillo Arianna, Marrapodi Maddalena, Capristo Carlo, Gicchino Maria Francesca, Montaldo Paolo, Caredda Elisabetta, Reibaldi Michele, Boatti Lara M V, Dell'Annunziata Federica, Folliero Veronica, Galdiero Marilena
机构信息
Department of Surgical Sciences, Eye Clinic Section, University of Turin, 10124 Turin, Italy.
Pediatric Unit, Fondazione IRCCS "Ca' Granda-Ospedale Maggiore-Policlinico", 20122 Milan, Italy.
出版信息
Microorganisms. 2022 Jul 10;10(7):1390. doi: 10.3390/microorganisms10071390.
UNLABELLED
The ocular microbiome is of fundamental importance for immune eye homeostasis, and its alteration would lead to an impairment of ocular functionality. Little evidence is reported on the composition of the ocular microbiota of term infants and on the impact of antibiotic prophylaxis.
METHODS
A total of 20 conjunctival swabs were collected from newborns at birth and after antibiotic treatment. Samples were subjected to 16S rRNA sequencing via system MiSeq Illumina. The data were processed with the MicrobAT software and statistical analysis were performed using two-way ANOVA.
RESULTS
Antibiotic prophylaxis with gentamicin altered the composition of the microbiota. In detail, a 1.5- and 2.01-fold reduction was recorded for () and (), respectively, whereas an increase in spp. of 6.5 times occurred after antibiotic exposure.
CONCLUSIONS
Antibiotic prophylaxis altered the ocular microbiota whose understanding could avoid adverse effects on eye health.
未标注
眼部微生物群对于眼部免疫稳态至关重要，其改变会导致眼部功能受损。关于足月儿眼部微生物群的组成以及抗生素预防的影响，报道的证据很少。
方法
在出生时和抗生素治疗后，从新生儿中总共采集了20份结膜拭子。通过Illumina MiSeq系统对样本进行16S rRNA测序。数据用MicrobAT软件处理，并使用双向方差分析进行统计分析。
结果
庆大霉素预防性使用改变了微生物群的组成。具体而言，（某菌属）和（另一菌属）分别减少了1.5倍和2.01倍，而抗生素暴露后（某菌属）增加了6.5倍。
结论
抗生素预防改变了眼部微生物群，了解这一点可以避免对眼部健康产生不利影响。
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