School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai 201203, China.
Molecules. 2022 Jul 15;27(14):4532. doi: 10.3390/molecules27144532.
Two novel natural flavonoid substituted polysaccharides (MBAP-1 and MBAP-2) were obtained from Lour. and characterized by HPGPC, methylation, ultra-high-performance liquid chromatography-ion trap tandem mass spectrometry (UPLC-IT-MS), and NMR analysis. The results showed that MBAP-1 was a homogenous heteropolysaccharide with a backbone of 4)-β-d-Glc-(1→ and →3,4,6)-β-d-Glc-(1→. MBAP-2 was also a homogenous polysaccharide which possessed a backbone of →3)-α-d-Glc-(1→, →4)-β-d-Glc-(1→ and →3,4)-β-d-Glc-2-OMe-(1→. Both the two polysaccharides were substituted by quercetin and exhibited anticomplement activities in vitro. However, MBAP-1 (CH: 0.075 ± 0.004 mg/mL) was more potent than MBAP-2 (CH: 0.249 ± 0.006 mg/mL) and its reduced product, MBAP-1R (CH: 0.207 ± 0.008 mg/mL), indicating that multiple monosaccharides and uronic acids might contribute to the anticomplement activity of the flavonoid substituted polysaccharides of . Furthermore, the antioxidant activity of MBAP-1 was also more potent than that of MBAP-2. In conclusion, these two flavonoid substituted polysaccharides from were found to be potential oxidant and complement inhibitors.
从 Lour.中得到了两种新型天然黄酮取代多糖（MBAP-1 和 MBAP-2），并通过 HPGPC、甲基化、超高效液相色谱-离子阱串联质谱（UPLC-IT-MS）和 NMR 分析进行了表征。结果表明，MBAP-1 是一种均一的杂多糖，其主链为 4)-β-d-Glc-(1→和 →3,4,6)-β-d-Glc-(1→。MBAP-2 也是一种均一的多糖，其主链为 →3)-α-d-Glc-(1→、→4)-β-d-Glc-(1→和 →3,4)-β-d-Glc-2-OMe-(1→。这两种多糖都被槲皮素取代，并具有体外抗补体活性。然而，MBAP-1（CH：0.075±0.004mg/mL）比 MBAP-2（CH：0.249±0.006mg/mL）及其还原产物 MBAP-1R（CH：0.207±0.008mg/mL）更有效，表明多个单糖和糖醛酸可能有助于 的黄酮取代多糖的抗补体活性。此外，MBAP-1 的抗氧化活性也强于 MBAP-2。总之，从 Lour.中得到的这两种黄酮取代多糖被发现是潜在的氧化剂和补体抑制剂。
