College of Food and Bioengineering, South China University of Technology, Guangzhou 510640, China.
College of Food and Bioengineering, South China University of Technology, Guangzhou 510640, China; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
Carbohydr Polym. 2021 Oct 1;269:118331. doi: 10.1016/j.carbpol.2021.118331. Epub 2021 Jun 11.
This study aimed to extract polysaccharides from citron and analyze their structures and potential bioactivities. Two novel polysaccharides CM-1 and CM-2 were purified from citron by DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography. Monosaccharide composition, linkage and NMR data were used to infer their sugar chains composition. The anti-breast cancer cells and immunoregulatory activities of CM-1 and CM-2 were investigated. Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position. While CM-2 (Mw = 22,303 Da), composed of arabinose, mannose, glucose and galactose in a molar ratio of 25.46:1.45:1.00:6.57, was galactoarabinan (GA) with (1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions. Both polysaccharides exhibited potential inhibiting cancer and immunostimulatory activities in vitro, especially CM-1. These results provide a basis for further research on citron polysaccharides.
本研究旨在从香橼中提取多糖，并分析其结构和潜在的生物活性。通过 DEAE-Sepharose Fast Flow 和 Sephadex G-100 柱层析从香橼中纯化得到两种新型多糖 CM-1 和 CM-2。通过单糖组成、连接方式和 NMR 数据推断其糖链组成。研究了 CM-1 和 CM-2 对乳腺癌细胞的抑制作用和免疫调节活性。结果表明，CM-1（Mw=21520Da）由阿拉伯糖、木糖、甘露糖和葡萄糖以摩尔比 10.78:11.53:1.00:1.70 组成，是阿拉伯木聚糖（AX），具有（1→4）-连接的β-d-Xylp 骨架，单取代有α-l-Araf 单元在 O-3 位。而 CM-2（Mw=22303Da）由阿拉伯糖、甘露糖、葡萄糖和半乳糖以摩尔比 25.46:1.45:1.00:6.57 组成，是半乳阿拉伯聚糖（GA），具有（1→5）-连接的α-l-Araf 主链，在 O-2 和/或 O-3 位被β-d-Galp 单元取代。这两种多糖都表现出体外抑制癌症和免疫刺激活性的潜力，尤其是 CM-1。这些结果为进一步研究香橼多糖提供了依据。
