Institute of Natural Products and Cosmetics, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Lodz, Poland.
Institute of Molecular and Industrial Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Lodz, Poland.
Molecules. 2022 Jul 19;27(14):4609. doi: 10.3390/molecules27144609.
plant is used in traditional Mongolian medicine. However, its chemical composition and biological properties are poorly explored. In this study, the total content of polyphenols and flavonoids as well as antioxidant activity were verified in plant extract. The total phenolic and flavonoid contents were determined by spectrometric (6.62 mg GAE/g and 10.32 mg QE/g) and chromatographic (17,598 mg/kg and 17,467 mg/kg) assays. The antioxidant potential was investigated by DPPH assay and yielded IC at 18.76 µg/mL. Twelve phenolic compounds were identified as components of extract. Kaempferol-3--robinosyl-7--rhamnoside and kaempferol-3-(-coumaroyl)-rutinosyl-7-rhamnoside made up 80% of determined components and were found to be the major polyphenolic compounds. The biological properties of extracts were determined in vitro using human epithelial adenocarcinoma Caco-2 cell line. Low concentrations of extract (0-30 µg/mL) exhibited protective effects against cell damage caused by chemically induced oxidative stress. Elevated concentrations, on the other hand, resulted in apoptotic-type cell death induction. Metabolic failure, ROS elevation and membrane permeabilization observed in cells upon incubation with extract dosages above 50 µg/mL allowed us to conclude on being predominantly a necrosis inducer.
该植物被用于传统蒙药中。然而，其化学成分和生物特性尚未得到充分研究。在本研究中，我们验证了植物提取物中的多酚和类黄酮总量以及抗氧化活性。通过分光光度法（6.62mgGAE/g 和 10.32mgQE/g）和色谱法（17,598mg/kg 和 17,467mg/kg）测定了总酚和总黄酮含量。通过 DPPH 测定法研究了抗氧化潜力，IC 为 18.76μg/mL。鉴定出 12 种酚类化合物作为提取物的成分。山奈酚-3-O-芸香糖苷和山奈酚-3-（咖啡酰基）-芦丁基-7-O-鼠李糖苷占测定成分的 80%，是主要的多酚类化合物。通过人上皮腺癌 Caco-2 细胞系在体外测定了 提取物的生物学特性。低浓度的提取物（0-30μg/mL）对化学诱导的氧化应激引起的细胞损伤表现出保护作用。另一方面，高浓度的提取物则导致凋亡型细胞死亡诱导。在孵育提取物剂量高于 50μg/mL 的细胞时观察到的代谢衰竭、ROS 升高和膜通透性，使我们得出结论，该植物主要是一种坏死诱导剂。
