Fresnillo Patricia, Jover-Gil Sara, Samach Alon, Candela Héctor
The Robert H. Smith Faculty of Agriculture, Food and Environment, The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
Instituto de Bioingeniería, Universidad Miguel Hernández, Campus de Elche, 03202 Elche, Spain.
Plants (Basel). 2022 Jul 13;11(14):1838. doi: 10.3390/plants11141838.
We report the first complete genome sequence of an isolate of (PaCV), a member of the family, identified in passion fruit ( Sims) plants grown in Israel. The assembled genome is 9672 nucleotides long and encodes a 3084 amino acids polyprotein that is predicted to be proteolytically cleaved into 10 mature peptides. Our analysis of the genome sequence shows that PaCV is a distinct species, sharing 68.5% nucleotide sequence identity and 71.5% amino acid sequence identity with isolates of the bean common mosaic necrosis virus (BCMNV), the most closely related virus classified within the genus . Using quantitative PCR, we detected the virus in RNA samples from leaves exhibiting symptoms of infection, with higher levels in clearly chlorotic leaves, but not in those from healthy leaves.
我们报道了从以色列种植的西番莲（西番莲属）植株中鉴定出的一种番木瓜环斑病毒（PaCV）分离株的首个完整基因组序列，该病毒属于花椰菜花叶病毒科。组装后的基因组长度为9672个核苷酸，编码一个3084个氨基酸的多聚蛋白，预计该多聚蛋白会被蛋白酶切割成10个成熟肽段。我们对基因组序列的分析表明，PaCV是一个独特的物种，与豆普通花叶坏死病毒（BCMNV）分离株的核苷酸序列同一性为68.5%，氨基酸序列同一性为71.5%，BCMNV是花椰菜花叶病毒属中关系最密切的分类病毒。通过定量PCR，我们在表现出感染症状的叶片RNA样本中检测到了该病毒，在明显黄化的叶片中病毒含量更高，但在健康叶片中未检测到。
